中国人兽共患病学报2017，Vol.33Issue(10)：897-902,6.DOI:10.3969/j.issn.1002-2694.2017.10.009
深圳地区四家医院感染性腹泻来源沙门菌耐药性及分子分型研究
Characteristics of drug resistance and molecular typing for Salmonella in diarrhea patients from four hospitals in Shenzhen
摘要
Abstract
We analyzed the antimicrobial resistance and pulsed field gel electrophoresis (PFGE) patterns of Salmonella strains of infectious diarrhea isolated four hospitals in Shenzhen during 2012-2016.A total of 137 Salmonella strains collected in 4 hospitals in Shenzhen were identified by serotyping,micro-dilution method was used to detect the antimicrobial susceptibility of the isolates.Pulsed field gel-electrophoresis(PFGE) was used for molecular typing and cluster analysis of S.typhimurium and S.enterica strains.Results showed that 137 strains of Salmonella were divided into 30 kinds of serotype.S.typhimurium was the predominant serotype.The drug susceptibility test indicated that the antibiotic resistance of the strains to chloromycetin,ampicillin and amoxicillin were more serious than other drugs,the resistance to penicillins,cephalosporins,aminolycosides and macrolides showed increase trends year by year.Of 49 strains of S.typhimurium were divided 38 molecular patterns by PFGE,with similarity ranged from 65.5％ 100％,TY18 was the main PFGE pattern.The 27 strains of S.enterica were divided into 7 molecular patterns by PFGE,with similarity ranged from 61.8％-100％,EN7 was the main PFGE pattern.The status of drug resistance of infectious diarrhea isolates was rather severe,PFGE patterns showed diversity,suggesting the diarrhea cases were distributed sporadically.Partial PFGE patterns and its corresponding antidrug spectrum have certain aggregation relationship.关键词
沙门菌/耐药特征/脉冲场凝胶电泳Key words
Salmonella/antimicrobial resistance character/pulsed field gel-electrophoresis引用本文复制引用
龙冬玲,韦洁宏,吴劲松,郝红雨,蒋淑萍,黎剑华,卓菲..深圳地区四家医院感染性腹泻来源沙门菌耐药性及分子分型研究[J].中国人兽共患病学报,2017,33(10):897-902,6.基金项目
深圳罗湖区软科学研究计划项目(No.2015035)资助.Supported by the Shenzhen Luohu District Research Project of Soft Science (No.2015035) （No.2015035）
