中国水产科学2017，Vol.24Issue(6)：1184-1192,9.DOI:10.3724/SP.J.1118.2017.17123
栉孔扇贝sox9基因的cDNA克隆及其在不同发育时期性腺中的表达特征
Molecular cloning of sox9 cDNA and its expression characteristics in gonads at different developmental stages of Chlamys farreri
摘要
Abstract
SOX9 is a member of the SOXE group in the SOX family, which plays an important role in sex deter-mination and differentiation of vertebrates. sox9 is specifically expressed in the testes of mammals, birds, and rep-tiles, and is expressed in both the testes and ovaries in amphibians and fishes. To measure the expression of sox9 in the gonads of invertebrates, we cloned a 2835 bp full-length cDNA of the scallop Chlamys farreri sox9 (Cf-sox9) which contains a 1413 bp open reading frame (ORF) encoding a 470-amino acid sequence with the HMG-box of the SOX family and the highly conserved region of the SOXE group, although there is no Pro-Gln- Ser-rich region at the C-terminus of vertebrate SOXE proteins. Cf-sox9 DIG-labeled RNA probes and Cf-SOX9 polyclonal anti-bodies were produced based on the Cf-sox9 full-length cDNA sequence. ISH and immunohistochemistry detection showed that Cf-sox9 transcripts and Cf-SOX9 proteins were located in all germ cells of C. farreri testes and ova-ries at different developmental stages. The expression patterns between sox9 mRNA and SOX9 protein were simi-lar. In the testes, the intensity of positive signals was highest in the spermatocytes and lowest in the spermatozoa. In the ovaries, the intensity of positive signals gradually decreased across the oogonia, oocytes, and mature oo-cytes. The expression characteristics of Cf-sox9 in C. farreri gonads were different from that most of vertebrates, which show variability in expression between sex, suggesting its function of gonad development and gametogene-sis might be different from vertebrates.关键词
栉孔扇贝/sox9/性腺/配子发生Key words
Chlamys farreri/sox9/gonad/gametogenesis分类
生物科学引用本文复制引用
梁少帅,于潇含,杨丹丹,秦贞奎..栉孔扇贝sox9基因的cDNA克隆及其在不同发育时期性腺中的表达特征[J].中国水产科学,2017,24(6):1184-1192,9.基金项目
国家 863 计划项目(2012AA10A402) （2012AA10A402）
山东省自然科学基金资助项目(ZR2016CQ24). （ZR2016CQ24）
