中国水产科学2017，Vol.24Issue(6)：1203-1212,10.DOI:10.3724/SP.J.1118.2017.17130
紫外辐射灭活大黄鱼和黄姑鱼精子及其激活大黄鱼卵子发育为胚胎的效果
Effects of UV irradiation on motility of sperm in Larimichthys crocea and Nibea albiflora and development of fertilized oocytes in L. crocea
摘要
Abstract
This study investigated the effects of UV irradiation on sperm motility and duration of sperm activity and determined the optimal UV irradiation dosage to genetically inactivate Larimichthys crocea and Nibea albi-flora sperm, while retaining adequate motility to fertilize L. crocea eggs. Milt from several males was diluted 1 : 30 with Ringer's solution and UV irradiated at an intensity of 2200 μW/(cm2·s), and used to fertilize L. crocea eggs. The fertilized eggs were divided into a cold-shock group (3℃ for 10 min at 2 min 30 s after fertilization) and a control group without cold-shock. Early embryo survival rate and larvae hatching rate were measured and compared. The results showed that: 1) motility of L. crocea and N. albiflora sperm was negatively correlated with UV treatment time, and the rapid changes in sperm showed a typical Hertwig effect; 2) early embryo survival rate induced by L. crocea and N. albiflora sperm in the control groups was negatively correlated with the UV exposure time, while the larvae hatching rate showed a typical Hertwig effect; 3) early embryo survival rate and larvae hatching rate induced by L. crocea and N. albiflora sperm in the cold-shock group showed a typical Hertwig effect with the increase in UV irradiation time, reaching peaks at 2 min 20 s and 1 min 30 s, respectively (the peak values of the survival and hatching rates of L. crocea were (38.3±4.3)% and (66.5±5.1)%, respectively; the peak values of the survival and hatching rates of N. albiflora were (43.3±3.3)% and (67.7±6.3)%, respectively). The results of this study indicated that the optimal UV dosages for L. crocea and N. albiflora sperm to activate L. crocea eggs were 308 mJ/cm2 and 198 mJ/cm2, respectively. These results provide information for the improvement of gyno-genetic technology for L. crocea.关键词
大黄鱼/黄姑鱼/紫外照射/精子活力/胚胎成活率/孵化率Key words
Larimichthys crocea/Nibea albiflora/UV irradiation/motility/survival rate/hatching rate分类
生物科学引用本文复制引用
汪倩凤,沈伟良,刘成,王迪,侯聪聪,竺俊全,吴雄飞..紫外辐射灭活大黄鱼和黄姑鱼精子及其激活大黄鱼卵子发育为胚胎的效果[J].中国水产科学,2017,24(6):1203-1212,10.基金项目
宁波市科技计划重大项目(2015C110005) （2015C110005）
浙江省科技计划重大项目(2016C02055-7). （2016C02055-7）
