中国水产科学2017，Vol.24Issue(6)：1245-1253,9.DOI:10.3724/SP.J.1118.2017.16386
应用高通量测序技术分析拟穴青蟹肠道及其养殖环境菌群结构
Application of high-throughput sequencing techniques for analyzing bacterial communities in pond-raised mud crab (Scylla paramamosain) intestine and its aquaculture environment
摘要
Abstract
Mud crab (Scylla paramamosain), widely cultured in brackish and seawater ponds along the coast of southern China, has become the most productive seawater crab species because of its strong adaptive abilities and high economic value. Bacteria play an important role in pond aquaculture ecosystems; however, little is known about the bacterial composition and diversity in earthen aquaculture pond microbial communities of S. parama-mosain. Accordingly, we applied Illumina HiSeq high-throughput sequencing to investigate bacterial community structure and diversity in pond-raised S. paramamosain intestinal and its aquacultural environment. The results showed that 234575 effective sequences were detected in three samples, and they could be classified into 2812 operational taxonomic units (OTUs), among which 453, 706, and 2547 OTUs belong to the gut of S. paramamo-sain, the aquaculture water, and the sediment, respectively. 184 OTUs were shared among all samples, 197 OTUs between S. paramamosain intestine and aquaculture water, 309 OTUs between S. paramamosain intestine and sediment, and 572 OTUs between the aquaculture water and sediment. This indicates that bacterial richness and diversity were lower in S. paramamosain intestine than in water and sediment. In total, 48 phyla were identified, among them Proteobacteria (39.96%), Tenericutes (23.09%), Firmicutes (16.58%), CKC4 (6.55%), and Bacter-oidetes (4.96%), which constituted the predominant components of the S. paramamosain intestine; Proteobacteria (63.02%), Actinobacteria (24.96%), Bacteroidetes (8.41%), Cyanobacteria (1.19%), and Firmicutes (0.83%) were the predominant phyla in the water; and the predominant phyla in the sediment were Proteobacteria (75.23%), Bacteroidetes (5.72%), Actinobacteria (3.83%), Chloroflex I (2.65%), and Acidobacteria (1.80%). Analysis of the 10 most abundant bacterial OTUs in different samples revealed that the most dominant bacteria in S. paramamo-sain intestine and its aquacultural environment were totally different, and there were only four kinds of dominant bacteria that have close sequences in the SILVA database. These results suggest that there exists a close correlation between bacterial species composition in the S. paramamosain intestine and its aquacultural environment. Mean-while, intestinal microflora may be a relatively independent microbial ecosystem and the micro-ecosystem could hardly be influenced by the bacteria in the culture environment.关键词
拟穴青蟹/肠道/养殖水体/底泥/菌群结构/高通量测序/微生态调控Key words
Scylla paramamosain/intestine/pond water/sediment/bacterial community/high-throughput se-quencing/microalgae ecology control分类
农业科技引用本文复制引用
王贤丰,赵艳飞,宋志飞,钟声平,黄国强,童潼,聂振平,苏琼,杨家林..应用高通量测序技术分析拟穴青蟹肠道及其养殖环境菌群结构[J].中国水产科学,2017,24(6):1245-1253,9.基金项目
广西科技开发项目(桂科合 1599005-2-18 （）
桂科能 1598020-10 （）
桂科 AB16380105 （）
桂科合 14125008-2-20). （）
