中国水稻科学2017，Vol.31Issue(6)：559-567,9.DOI:10.16819/j.1001-7216.2017.7070
水稻OsHKT基因表达模式分析
Expression Patterns of OsHKT Genes in Rice
摘要
Abstract
[Objective]OsHKTs (High Affinity K+ Transporter) encode Na+ or K+ transporter or Na+-K+ co-transporters that play an important role in Na+ recirculation and maintaining the low concentration of Na+ and low Na+ / K+ ratio in above-ground tissues, especially leaves. Therefore, OsHKT family is related to salt stress tolerance in rice. In this study, we analyzed the tissue specificity and diurnal rhythm of OsHKTs gene expression and expression patterns in rice seedlings treated with either salt or ABA.[Method]qRT-PCR technique was used to analyze OsHKTs expression patterns.[Result]Individual member of OsHKT family genes had different expression levels in root and leaf. OsHKT1;1, OsHKT1;3, OsHKT2;3, and OsHKT2;4 mainly expressed in leaves, whereas other members had higher transcript levels in roots relative to those in leaves. Salt treatment affected transcript levels of OsHKT family genes both in leaves and roots. OsHKT1;5, OsHKT2;1, and OsHKT2;2 had similar expression patterns in both leaves and roots of seedlings treated with NaCl, whereas other members did not. Transcript levels of OsHKT family genes were regulated by ABA treatment either in leaves or in roots. OsHKT1;3 and OsHKT1;5 had similar expression patterns in both leaves and roots. In addition, expression patterns of OsHKT genes exhibited similar diurnal rhythm. [Conclusion]Our results suggest that expression of OsHKT family genes has tissue-specificity and similar diurnal rhythm. Exogenous NaCl and ABA treatment have different influences on OsHKT transcripts, depending on individual member of the family. Our findings lay a foundation for further revealing the functional mechanism of OsHKT genes in rice.关键词
OsHKT/表达模式/盐胁迫/ABA/组织特异性/光周期Key words
OsHKT/expression patterns/salt stress/ABA/tissue specificity/photoperiod分类
农业科技引用本文复制引用
崔立新,和亚男,李亚萍,谢先芝..水稻OsHKT基因表达模式分析[J].中国水稻科学,2017,31(6):559-567,9.基金项目
山东省科技重大专项(2015ZDJS03001-4) （2015ZDJS03001-4）
山东省中青年科学家科研奖励基金资助项目(ZR2016CB17) （ZR2016CB17）
山东省农业重大应用技术创新项目[黄河三角洲中高度盐碱地水稻品种(系)筛选与淡水控量增效技术研究与示范]. （系）
