中国水稻科学2017，Vol.31Issue(6)：631-642,12.DOI:10.16819/j.1001-7216.2017.6168
粳稻种质资源芽期耐盐性综合评价与筛选
Comprehensive Evaluation and Selection of Rice (Oryza sativa japonica) Germplasm for Saline Tolerance at Germination Stage
摘要
Abstract
[Objective]Soil salinity is one of the major abiotic stresses affecting rice growth and production. Salt resistance identification at germination stage, indices screening, and cultivar breeding of rice germplasm resources are important for rice production.[Method]Seeds of sixty-four japonica rice germplasm accessions germinated in the 125 mmol L-1 NaCl solution. On the third day after salt stress, germination number of the seeds was measured. After salt stress for five and ten days, germination number, shoot length and root length were measured and then the relative shoot length, relative root length, relative germination potential, relative germination rate, relative salt damage rate, germination index and vigor index were calculated. Multiple statistical methods were used to comprehensively evaluate the salt tolerance of 64 rice japonica germplasm and the germination characteristics of typical salt-tolerant and salt-sensitive rice germplasm under salt stress were analyzed.[Result]The relative salt damage rate was significantly negatively correlated with relative root length, relative germination potential, relative germination rate, germination index and vigor index. Except for relative shoot length, these six indexes were significantly correlated with each other. Through cluster analysis, 64 japonica rice germplasm were divided into four groups. The groupⅠ andⅣ were typical salt-sensitive and salt-tolerant germplasm, respectively. The group Ⅱ was mainly composed of low salt-resistant rice and the group Ⅲ was moderately salt-tolerant. With the principal component analysis these seven single indexes could be converted to three principal components. An objective comprehensive evaluation value (D value) of salt tolerance was obtained using membership function with index weight method. The five highest and lowest D value germplasm resources were selected to carry on significance analysis. The results showed that except for relative shoot length, all of these seven indexes measured after stress for five and ten days were significantly different from the two types. [Conclusion]Rice was sensitive to salt stress at germination stage and there were significant differences between different germplasm. Using stepwise regression and principal component analysis, germination index, relative root length and relative salt damage rate were selected as the key indexes for the rapid identification of salt tolerance of rice germplasm. If multivariate statistical method was used, the reliability will be improved.关键词
水稻/种质资源/芽期耐盐性/主成分分析/综合评价Key words
rice/germplasm resource/salt tolerance at germination stage/principal component analysis/comprehensive evaluation分类
农业科技引用本文复制引用
田蕾,胡慧,张银霞,李培富,陈亚萍,刘俊,马晓刚,王娜,杨兵,李莹,郭海东,李娟..粳稻种质资源芽期耐盐性综合评价与筛选[J].中国水稻科学,2017,31(6):631-642,12.基金项目
国家自然科学基金资助项目(31401361) （31401361）
宁夏农业育种专项(2013NYYZ0302). （2013NYYZ0302）
