中国水稻科学2017，Vol.31Issue(6)：643-651,9.DOI:10.16819/j.1001-7216.2017.7012
共生菌Arsenophonus、水稻品种和温度对褐飞虱黄绿绿僵菌发病率的影响
Influence of Symbiotic Bacteria Arsenophonus, Rice Variety and Temperature on the Incidence Rate of Nilaparvata lugens to Metarhizium flavoviride
摘要
Abstract
[Objective] To reveal the significant factors affecting the incidence rate of the brown planthopper (BPH) to Metarhizium flavoviride, laboratory bioassays were carried out to study the influence of symbiotic bacteria Arsenophonus, rice variety and temperature on the incidence rate of BPHs to M. flavoviride. [Method] Through two completely randomized block trial: 1) symbiotic bacteria Arsenophonus (infected and uninfected) versus rice varieties (TN1, IR56 and Mudgo), 2) and Arsenophonus (infected and uninfected) versus temperatures (21°C, 23°C, 25°C, 27°C, 29°C and 31°C), BPHs with symptoms of M. flavoviride infection were observed after exposure to the spore suspensions of M. flavoviride for different periods.[Result]For all tested rice varieties, the incidence rates of M. flavoviride-treated BPHs infected with Arsenophonus (BPH++) were lower than that of BPHs uninfected (BPH--). Out of tested temperatures, only under 25°C, 27°C, 29°C, BPH++ has lower incidence rates than BPH-- at 3 d and 5 d after spraying. However, there was no significant difference among others. The results of two-way ANOVA analysis between Arsenophonus and rice varieties also indicated that the incidence rate of BPH++ was lower than that of BPH--. Furthermore, two-way ANOVA results between Arsenophonus and temperatures indicated the incidence rate of BPH++ was lower than that of BPH-- at 3 d and 5 d after spraying. The effect of temperature was significant on the virulence of M. flavoviride to BPHs. BPHs had higher corrected incidence rates and shorter LT50 under 23°C-29°C than that under 21°C or 31°C. Out of these BPHs, the highest corrected incidence rates and shortest LT50 were found at 27°C. It suggested 23°C-29°C was suitable temperature for infection of M. flavoviride, among which 27°C was the best. This suggested that low or high temperatures would negatively affect the virulence of M. flavoviride to BPH. The temperature also affected the LT50 of BPHs. The LT50 of BPH++ was longer than that of BPH-- under 27°C or 29°C. However, the LT50 of BPH++ was equal or even shorter than that of BPH-- under other temperatures.[Conclusion] Arsenophonus decreased the virulence of M. flavoviride to BPH, which was influenced by temperature. The temperature also affects the incidence rate of BPHs to Metarhizium flavoviride significantly. However, the effects of rice varieties and interactions of Arsenophonus and rice varieties and Arsenophonus and temperatures were not significant on the incidence rate.关键词
褐飞虱/黄绿绿僵菌/Arsenophonus/温度/水稻品种Key words
Nilaparvata lugens/Metarhizium flavoviride/Arsenophonus/temperature/rice variety分类
农业科技引用本文复制引用
朱欢欢,陈洋,万品俊,王渭霞,赖凤香,傅强..共生菌Arsenophonus、水稻品种和温度对褐飞虱黄绿绿僵菌发病率的影响[J].中国水稻科学,2017,31(6):643-651,9.基金项目
国家自然科学基金资助项目(31401738) （31401738）
中央级公益性科研院所基本科研业务费专项(2014RG005) （2014RG005）
农业现代产业技术体系资助项目(CARS-1-18) （CARS-1-18）
中国农业科学院科技创新工程水稻病虫草害防控技术创新团队项目. （）
