中国水稻科学2017，Vol.31Issue(6)：652-657,6.DOI:10.16819/j.1001-7216.2017.7004
水稻干尖线虫Ab-lea基因在高渗透压下的表达
Expression Under Hypertonic Osmotic Stress of Ab-lea from Aphelenchoides besseyi
摘要
Abstract
[Objective]Rice white tip nematodes (Aphelenchoides besseyi) can enter osmobiosis to survive hypertonic osmotic stress in soil and seeds before infecting hosts, making the prevention and control of the nematode rather difficult. Normally, under hypertonic osmotic stress, the gene expression of late embryogenesis abundant protein (LEA) will significantly increase. As an adversity expressive protein, LEA can protect the organism under adversity. In order to study the expression pattern and the function of LEA gene from A. besseyi (Ab-lea) under hypertonic osmotic stress,[Method]the gene was cloned according to the results of the transcriptome sequencing. A saturated MgSO4·7H2O solution was used as a hypertonic osmotic solution to dehydrate nematodes. The expression of Ab-lea was tested by RT-PCR during Osmobiosis. RNAi was used to investigate the survival of nematodes under hypertonic osmotic stress after silencing of Ablea.[Result]Nematodes will turn into osmobiosis after soaking in a saturated MgSO4·7H2O solution for 240 min. RT-PCR results showed that the expression of Ab-lea was upregulated when nematodes were at the beginning of hypertonic osmotic stress treatment and during the osmobiosis. [Conclusion]The survival rate of the nematodes dropped significantly under osmobiosis after Ab-lea silence, which showed that Ab-lea may be involved in the regulation of osmobiosis of the nematode. It lays a foundation of further exploration for the physiological mechanism of nematode-resistance to hypertonic osmotic stress and provides new ideas for the prevention and control of the nematode.关键词
胚胎发育晚期丰富蛋白/水稻干尖线虫/高渗透压胁迫/变渗隐生/基因干涉Key words
late embryogenesis abundant protein/Aphelenchoides besseyi/hypertonic osmotic stress/osmobiosis/RNAi分类
农业科技引用本文复制引用
陈俏丽,王峰,李丹蕾,零雅茗,张瑞芝..水稻干尖线虫Ab-lea基因在高渗透压下的表达[J].中国水稻科学,2017,31(6):652-657,6.基金项目
国家自然科学基金资助项目(31570637) （31570637）
