中国现代医学杂志2017，Vol.27Issue(27)：1-6,6.DOI:10.3969/j.issn.1005-8982.2017.27.001
五味子乙素抗肾纤维化作用及机制研究
Protective effect of Schisandrin B on renal fibrosis and underlying mechanism
摘要
Abstract
Objective To investigate the protective of Schisandrin (Sch B) on renal fibrosis and underlying mechanism. Methods Mice were subjected to ischemia-reperfusion (I/R) injury in presence or absence of Sch B. Masson staining was performed for morphological grading of renal fibrosis. α-SMA and Collagen I expression in kidney tissue were determined by Immunohistochemistry. The expression levels of α-SMA, collagenⅠ, E-cadherin, transforming growth factor β1 (TGF-β1) and p-Smad3 were measured by Western blot. Results Renal fibrosis and tubular atrophy were significantly alleviated by Sch B treatment when compared with I/R group. Immunohistochemistry staining showed that α-SMA and Collagen I in the I/R group increased significantly, which was attenuated by Sch B. Western blot suggested that, in the Sch B group, the expression of E-cadherin was increased while α-SMA, Collagen I, TGF-β1 and p-Smad3 decreased dramatically in a dose dependent manner in comparison with those in the I/R group. Conclusions Data confirms that SchB can ameliorate renal fibrosis by inhibiting TGF-β/Smad signaling pathway, which could be a potential therapeutic intervention for renal fibrosis.关键词
肾纤维化/五味子乙素/缺血再灌注/治疗Key words
renal fibrosis/Schisandrin B/ischemia reperfusion分类
医药卫生引用本文复制引用
郑海洲,张杰,肖程程,秦聪,徐涛..五味子乙素抗肾纤维化作用及机制研究[J].中国现代医学杂志,2017,27(27):1-6,6.基金项目
国家自然科学基金(No:81470923) （No:81470923）
