中国现代医学杂志2017，Vol.27Issue(27)：7-11,5.DOI:10.3969/j.issn.1005-8982.2017.27.002
草苁蓉环烯醚萜苷对二乙基亚硝胺诱发肝癌大鼠细胞凋亡的影响
Effect of Iridoid Glucosides from Boschniakia Rossica on rat models of diethylnitrosamine-induced hepatocarcinomat
摘要
Abstract
Objective To investigate the effect of Iri doid Glucosides from Boschniakia Rossica (IGBR) on apoptosis and the expression of p53 and Bcl-2 proteins. Methods A total of 132 Wistar male rats were randomly divided into control group, model group, positive control group and IGBR group. DEN was administered once by intraperitoneal injection (0.2 g/kg), then through drinking water (0.05%) for the rest of experimental duration in each group except for the control group. Animals from the positive control group was injected with 5-FU 3 times per week (0.025 g/kg). Rats in the IGBR group were treated intragastrically with IGBR daily (0.5 g/kg). Animals were sacrificed on 12, 20 or 28 weeks post insults. Pathological alterations and apoptosis of liver cells were observed and expression of p53 as well as Bcl-2 were determined by Immunohistochemistry and Western blot. Results Compared with the control group, the enlarged and deeply stained nuclei, hepatocellular degeneration, dysplasia and cancer cell were observed in the liver tissue of the model group. Apoptotic hepatocyte showed cytoplasmic and nuclear shrinkage without nucleolus. Immunohistochemistry results showed that p53 and Bcl-2 positive signals were mainly distributed in the cytoplasm of atypical hyperplasia and carcinoma. Expression of p53 increased while Bcl-2 decreased in the IGBR group as well as the positive control group significantly compared with the model group ( <0.05). No obvious differences were observed between the IGBR group and the positive control group ( > 0.05). Conclusions Hepatocarcinoma is closely associated with apoptosis, and IGBR inhibits DEN -induced hepatocarcinoma through mediating p53 and Bcl-2 pathway.关键词
肝癌/草苁蓉环烯醚萜苷/凋亡/p53/Bcl-2Key words
hepatocarcinoma/Iridoid Glucosides from Boschniakia Rossica (IGBR)/apoptosis/p53/Bcl-2分类
医药卫生引用本文复制引用
崔香丹,郑峰,朱洁波,全吉淑,尹学哲..草苁蓉环烯醚萜苷对二乙基亚硝胺诱发肝癌大鼠细胞凋亡的影响[J].中国现代医学杂志,2017,27(27):7-11,5.基金项目
国家自然科学基金(No:81360651) （No:81360651）
