中国现代医学杂志2017，Vol.27Issue(27)：12-16,5.DOI:10.3969/j.issn.1005-8982.2017.27.003
右美托咪定对神经病理性痛大鼠脊髓背角COX-2和c-fos表达的影响
Effect of Dexmedetomidine on spinal expression of COX-2 and c-fos in rat models of neuropathic pain
摘要
Abstract
Objective To investigate the effects of Dexmedetomidine on spinal expressions of cyclooxygenase-2 (COX-2) and c-fos in spinal dorsal horns of rat models of neuropathic pain. Methods A total of 90 healthy adult male Wistar rats were randomly divided into sham group ( =30), neuropathic pain group ( =30) and Dexmedetomidine group ( =30). Rat models of neuropathic pain were established by sciatic nerve ligation. Sham operated rats received all procedure except for sciatic nerve ligation. Dexmedetomidine was intraperitoneally injected (50μg/kg) daily in the Dexmedetomidine group. Vehicle saline were administered into the remaining animals. Thermal pain threshold (TWL) and mechanical pain threshold (MWT) were measured 1 day before operation (T0), and the 3th d (T1), 7th d (T2) and 14th d (T3) after surgery, then spine were harvested immediately. COX-2 and c-fos in spinal cord dorsal horns were determined by real-time quantitative PCR. Results Compared with the sham group, TWL and MWT in the neuropathic pain group and Dexmedetomidine group decreased significantly at T1~T3 ( <0.05); TWL and MWT in the Dexmedetomidine group increased. at T1~T3 ( <0.05) compared with the neuropathic pain group. Expression levels of COX-2关键词
右美托咪定/神经病理性痛/大鼠/环氧化酶-2/c-fosKey words
Dexmedetomidine/neuropathic pain/cyclooxygenase-2/c-fos分类
医药卫生引用本文复制引用
秦梦婷,邱忠鹏,王业忠..右美托咪定对神经病理性痛大鼠脊髓背角COX-2和c-fos表达的影响[J].中国现代医学杂志,2017,27(27):12-16,5.基金项目
国家自然科学基金(No:81360185) （No:81360185）
