中国现代医学杂志2017，Vol.27Issue(27)：17-21,5.DOI:10.3969/j.issn.1005-8982.2017.27.004
髓系抑制性细胞和调节性T细胞在小鼠实验性结肠炎中的作用
Role of myeloid-derived suppressor cells and regulatory T cells in mouse models of ulcerative colitis
摘要
Abstract
Objective To investigate the role of myeloid-derived suppressor cells (MDSC) and regulatory T (Treg) cells in development of ulcerative colitis. Methods Mouse models of ulcerative colitis were established with administration of 5% dextran sodium sulfate (DSS). Body weight and hematochezia were obtained daily. Mice were sacrificed 3 or 7 days post insult. Histological assessment of ulcerative colitis was graded with hematoxylin-eosin staining. The percentages of MDSC and Treg cells in the spleen and mesenteric lymph nodes were measured by flow cytometry. Results In animals treated with DSS, body weight loss, diarrhea and hematochezia were observed on the 4th day. Histological analysis revealed colonic mucosa damage and manifestation of inflammation. The amount of Treg cells was increased, whereas the amount of MDSC was decreased significantly in mesenteric lymph nodes in 7-day model group ( <0.05). Further observation on the subsets of MDSC suggested that the percentage of granulocyte-like MDSC decreased in the 7-day model group ( < 0.05) while no significant change of monocyte-like MDSC was observed among all the groups. There was no significant difference in the amount of Treg cells or MDSC in spleen among three groups. Conclusion Acute ulcerative colitis experiences decreased MDSC and increased Treg cells, which could contribute to the development of ulcerative colitis.关键词
溃疡性结肠炎/髓系抑制性细胞/调节性T细胞/免疫调控Key words
ulcerative colitis/myeloid-derived suppressor cell/regulatory T cell分类
医药卫生引用本文复制引用
吕颖,卢建华,吕卓,段媛媛,刘志权,崔美兰,闫会敏..髓系抑制性细胞和调节性T细胞在小鼠实验性结肠炎中的作用[J].中国现代医学杂志,2017,27(27):17-21,5.基金项目
河北省自然科学基金(No:H2015106081) （No:H2015106081）
河北省医学科学研究课题计划项目(No:20150893) （No:20150893）
