中国现代医学杂志2017，Vol.27Issue(27)：22-26,5.DOI:10.3969/j.issn.1005-8982.2017.27.005
参芍口服液对糖尿病肾病大鼠肾脏内质网应激因子GRP78、PERK及CHOP表达的影响
Effect of Shenshao oral liquid on expression of GRP78, PERK and CHOP in rat models of diabetic nephropathy
远迪 1董云朋 1曾招军 1黄安静 1张春来 2周洪霞2
作者信息
- 1. 华北理工大学,河北 唐山 063000
- 2. 河北省唐山工人医院 心内四科,河北 唐山 063000
- 折叠
摘要
Abstract
Objective To investigate the effect of Shenshao oral liquid on expression of endoplasmic reticulum stress factors GRP78, PERK and CHOP in rat models of diabetic nephropathy. Methods A total of 30 healthy male Wistar rats were randomly divided into Sham group, model group (DN group) and Shenshao oral liquid group (SS group). Animals in DN group and SS group were fed with high fat diet in addition to intraperitoneal injection of streptozotocin. Rats in SS group were administrated with Shenshao oral liquid (250 mg/kg) daily, and distilled water were administered daily into rats from Sham group and DN group. Blood glucose, blood urea nitrogen (BUN), creatinine (Scr) and 24-hour urinary protein (PRO) were measured 12 weeks post insults. Histopathological changes in renal tissue were graded through HE staining. Expression levels of GRP78, PERK and CHOP in kidney were measured by Western blot. Apoptosis rate were determined by TUNEL staining. Results Blood glucose, BUN, Scr and PRO in rats from DN group were significantly increased compared with Sham group ( <0.05), which were attenuated with treatment of Shenshao oral liquid ( <0.05). HE staining showed that morphological abnormality was observed in DN group including enlarged glomerular volume, inflammatory cell infiltration, glomerular mesangial stromal hyperplasia, tubular basement membrane thickening, and glomerular and tubular fibrosis. Above histopathological deterioration of renal tissue was significantly ameliorated by treatment of Shenshao oral liquid ( < 0.05). Western blot showed that the expression levels of GRP78, PERK and CHOP in renal tissue of DN group were significantly increased when compared with Sham group, which was decreased in SS group. TUNEL staining manifested that increased apoptosis rate in DN group ( <0.05) in comparison with Sham group could be attenuated in SS group ( <0.05). Conclusions Shenshao oral liquid reduces development of diabetic nephropathy, restoring renal function in dependence of manipulation on endoplasmic reticulum stress related factors GRP78, PERK, CHOP and apoptosis.关键词
参芍口服液/糖尿病肾病/内质网应激/细胞凋亡/PERK/大鼠Key words
shenshao oral liquid/diabetic nephropathy/endoplasmic reticulum stress/apoptosis分类
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远迪,董云朋,曾招军,黄安静,张春来,周洪霞..参芍口服液对糖尿病肾病大鼠肾脏内质网应激因子GRP78、PERK及CHOP表达的影响[J].中国现代医学杂志,2017,27(27):22-26,5.
