中国现代医学杂志2017，Vol.27Issue(27)：27-31,5.DOI:10.3969/j.issn.1005-8982.2017.27.006
下调基因表达对胃癌细胞增殖、迁移和侵袭的影响
Genetic inhibition of on proliferation, migration andα-catulin invasion of gastric cancer cells
摘要
Abstract
Objective To investigate the effect of genetic inhibition of α-catulin on proliferation, migration and invasion of gastric cancer cells. Methods Human gastric cancer cell line SGC-7901 were cultured and were divided into siRNA-α-catulin group, siRNA sequence control group and negative control group. MTT and Transwell assay were performed to measure cell proliferation and cell invasion, respectively. The levels ofα-catulin, N-cadherin, E-cadherin and Vimentin were determined by Western blot. Results MTT results indicated that optical density value at 24 h, 48 h, 72 h and 96 h in the siRNA-α-catulin group were significantly lower than that of siRNA-control sequence group and negative control group ( < 0.05). The amount of migrating cells and invasive cells in siRNA-α-catulin group were decreased dramatically compared with siRNA-control sequence group and blank control group ( <0.05). Expression levels of α-catulin, N-cadherin and Vimentin in siRNA-α-catulin group decreased while E-cadherin increased significantly when compared with siRNA-control sequence group and blank control group ( < 0.05). Conclusion Genetic knockdown of α-catulin gene in SGC-7901 cell line could prevent cell proliferation, migration and invasion by inhibition of transition of epithelial-mesenchymal.关键词
胃癌/α-catulin/细胞增殖/细胞侵袭/上皮-间质转化Key words
gastric cancer/α-catulin/cell proliferation/cell invasion/epithelial-mesenchymal transit分类
医药卫生引用本文复制引用
赵乃阔,胡庆军,史朝晖..下调基因表达对胃癌细胞增殖、迁移和侵袭的影响[J].中国现代医学杂志,2017,27(27):27-31,5.基金项目
河南省2015年科技发展计划(No:152300410164) （No:152300410164）
