中国医科大学学报2017，Vol.46Issue(7)：582-586,5.DOI:10.12007/j.issn.0258-4646.2017.07.002
纯化兔血清对氧磷酶1与传统方法对敌敌畏所致大鼠肝脏损伤的疗效比较
Use of Purified Rabbit Serum Paraoxonase 1 Compared with the Traditional Method of Curative Effect on Dichlorvos-induced Liver Injury in Rats
摘要
Abstract
Objective To explore whether the use of purified rabbit serum paraoxonase 1 (PON1) for the treatment of dichlorvos-induced liver injury in rats is superior to traditional method.Methods Thirty male SD rats were randomly divided into the followint 5 groups,with 6 rats in each group:control group (A group),dichlorvos group (B group),traditional treatment group (C group),PON 1 treatment group (D group),combined treatment group (E group).Rats in groups B,C,D and E were adminstered dichlorvos by intraperitoneal injection 9 mg/kg.In group C,atropine 10 mg/kg and iodine solution 45 mg/kg were injected intraperitoneally within 2 min after dichlorvos administration.In group D,PON1 was injected intravenously at a dose of 9 600 U/kg,30 min prior to poisoning.In group E,PON1 was injected intravenously at a dose of 9 600 U/kg,30 min prior to poisoning,followed by in travenous injection of atropine 10 mg/kg and iodine solution 45 rng/kg within 2 min after poisoning.Rats in A group received normal saline.Blood was collected at different time points to examine the acetyl cholinesterase (AChE)-levels by ELISA method.Liver tissue were collected at 12 hours after model establishment to observe the pathological changes.The expression of 4 hydroxy 2-nonenal (4-HNE) in the liver tissue was detected by immunohistochemistry and Western blotting.Results In group B,AChE levels decreased significantly,liver cells showed severn fatty degeneration,karyopyknosis and other pathological changes,and 4-HNE expression increased.The pathological changes of group D and group E were less obvious than those of group C,and the 4-HNE expression in the group D and group E were significantly different from that in the group C (P＜ 0.05).Conclusion PON1 plays a protective role in dichlorvos-induced liver injury in rats,and this protection is better than that offered by traditional treatment.关键词
对氧磷酶1/敌敌畏中毒/肝损伤/氧化应激/4-羟基壬烯醛Key words
paraoxonase 1/dichlorvos poisoning/liver injury/oxidative stress/4-hydroxy 2-nonenal分类
医药卫生引用本文复制引用
王聪,陶维晨,李琦,吴泽扬,郭镇豪,赵敏..纯化兔血清对氧磷酶1与传统方法对敌敌畏所致大鼠肝脏损伤的疗效比较[J].中国医科大学学报,2017,46(7):582-586,5.基金项目
国家自然科学基金(81671898) （81671898）
盛京自由研究者计划(201207) （201207）
