中国医科大学学报2017，Vol.46Issue(7)：595-599,5.DOI:10.12007/j.issn.0258-4646.2017.07.005
过表达microRNA-486对大鼠心肌梗死后心脏功能损伤的改善
Overexpression of microRNA-486 Improves the Cardiac Function in Rats with Myocardial Infarction
摘要
Abstract
Objective To investigate the effects of microRNA-486 (miR-486) expression on the cardiac function in rats with myocardial infarction (MI),through direct injection of an adenovirus carrying the miR-486 gene into the myocardium.Methods Totally 120 male rots were divided into 4 groups.Different target materials were infused into the surrounding areas of MI after building a successful disease model.Expression of miR-486 in the surrounding areas of left ventricular MI was detected using real-time PCR at different time points.Cardiac function was measured by cardiac ultrasound at 4 weeks.Using TUNEL,TTC,Masson,and CD31 staining to measure the organic changes in heart tissues.Results The expression of miR-486 in miR-486 group was higher than the other three groups.The expression of miR-486 in the three groups decreased when the time of MI in rats was prolonged.The cardiac function of the miR-486 group was better than that of the MI and AD groups.The MI area in the miR-486 group was smaller than that in the other two groups.Compared to the Sham group,the CVF at the infarct border zone was increased in the MI,Ad,and miR-486 groups.The AI in the MI and Ad groups was significantly increased compared to that in the miR-486 group.MVD was increased in the miR-486 group compared to that in the Sham,MI,and Ad groups.Conclusion miR-486 improved the cardiac function after MI in rats,through lightening collagen deposition,inhibiting apoptosis,and inducing angiogenesis in ischemic regions.关键词
心肌梗死/microRNA-486/心肌纤维化/心肌细胞凋亡/微血管新生Key words
myocardial infarction/microRNA-486/collagen/apoptosis/microvessel density分类
医药卫生引用本文复制引用
刘鹏飞,张海涛,齐弘炜,王楠楠,张博阳,袁彪,李田昌..过表达microRNA-486对大鼠心肌梗死后心脏功能损伤的改善[J].中国医科大学学报,2017,46(7):595-599,5.基金项目
国家自然科学基金(81370237) （81370237）
