中国医科大学学报2017，Vol.46Issue(7)：604-608,5.DOI:10.12007/j.issn.0258-4646.2017.06.007
冬凌草甲素诱导胶质瘤SHG44细胞凋亡及其分子机制的研究
Mechanism of Oridonin-induced Apoptosis of Glioma SHG44 Cells
摘要
Abstract
Objective To study the molecular mechanism of oridonin-induced apoptosis of ghoma SHG44 cells.Methods A growth curve was plotted using CCK-8 colorimetric method with different concentrations of oridonin (0,1.25,2.5,5,10,20,and 40 μmol/L)to observe its effect on the growth of SHG44 cells.Hoechst33258 and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining were used to examine the changes in cell morphology and flow cytometry was used to detect cell apoptosis.Western blotting was used to analyze the expression of apoptosis-related proteins (Caspase-3,cleaved Caspase-3,Bax,and Bcl-2)in SHG44 cells.Results SHG44 cell proliferation was significantly suppressed after 24 and 48 h Oridonin treatment,with a half-maximal inhibitory concentration of 7.865 and 4.74 μmol/L,respectively.Hoechst33258 and TUNEL staining showed changes in cell morphology such as shrinkage and nucleus fragmentation and morphogenesis,which are indicative of apoptosis.Western blotting analysis showed that oridonin inhibited the expression of Bcl-2 and activated the expression of Caspase-3 and Bax.Conclusion Oridonin can inhibit the proliferation and induce the apoptosis of SHG44 cells by regulating the expression of apoptosis-related proteins.关键词
冬凌草甲素/胶质瘤/SHG44细胞/细胞凋亡Key words
oridonin/glioma/SHG44 cells/cell apoptosis分类
医药卫生引用本文复制引用
朱莽,龙乾发,杨彦平,樊欣鑫,谢江涛,姜海涛..冬凌草甲素诱导胶质瘤SHG44细胞凋亡及其分子机制的研究[J].中国医科大学学报,2017,46(7):604-608,5.基金项目
国家自然科学基金(31301216) （31301216）
