中国医科大学学报2017，Vol.46Issue(7)：623-627,5.DOI:10.12007/j.issn.0258-4646.2017.07.011
Tim-1蛋白表达及其基因多态性与系统性红斑狼疮患者营养功能的相关性
Association of Tim-1 Protein Expression and Gene Polymorphism with Nutritional Function in Patients with Systemic Lupus Erythematosus
摘要
Abstract
Objective To investigate the association of Tim-1 protein expression and its gene polymorphism with nutritional parameters in patients with systemic lupus erythematosus (SLE).Methods Peripheral blood samples were collected from 126 patients with SLE.Serum Tim-1 protein levels were detected by ELISA,and the Tim-1 gene-416G＞C,-1454G＞A polymorphism was detected by PCR-RFLP.Prealbumin,ceruloplasmin,and retinol conjugated protein levels were determined by immunoturbidimetry.Ferritin and 1,25-dihydroxy vitamin D3 levels were detected by electrochemiluminescence.Results Concentrations of serum Tim-1 protein,prealbumin,ceruloplasmin,retinol binding protein,ferritin,and 1,25-dihydroxy vitamin D3 were 249.7±30.2 pg/mL,226±42 μg/mL,363±95 μg/mL,29.4± 13.2 μg/mL,355± 164 ng/mL,and 26.4-± 11.5 ng/mL,respectively.In the-416G＞C site,GG,GC,and CC genotypes accounted for 11.9％,57.1％,and 31.0％,respectively.In the-1454G＞A site,GG,GA,and AA genotypes accounted for 67.5％,26.2％,and 6.3％,respectively.The Tim-1 protein concentration did not differ significantly between the different genotypes of the-416G＞C site (F=0.575,P=0.564) or-1454G＞A site (F=1.255,P=0.289).Tim-1 level was significandy negatively correlated with prealbumin (r =-0.176,P =0.033),and positively correlated with ceruloplasmin (r =0.205,P =0.014) and 1,25-dihydroxy vitamin D3 (r=0.166,P=0.042).The serum prealbumin level decreased significantly (P=0.027) in patients harboring the GG genotype in the-1454G＞A site,whereas the serum 1,25-dihydroxy vitamin D3 level decreased significandy (P =0.024) in patients with the AA genotype in the-1454G＞A site.Conclusion Serum Tim-1 protein level and the-1454G＞A polymorphism of Tim-1 gene are associated with the nutritional function of patients with SLE.关键词
系统性红斑狼疮/Tim-1/基因多态性/营养功能Key words
systemic lupus erythematosus/Tim-1/gene polymorphism/nutritional function分类
医药卫生引用本文复制引用
陈向华,王建吉,耿学丽,丁萌,华正祥..Tim-1蛋白表达及其基因多态性与系统性红斑狼疮患者营养功能的相关性[J].中国医科大学学报,2017,46(7):623-627,5.基金项目
承德市科学技术研究与发展计划(201601A031) （201601A031）
