中国医科大学学报2017，Vol.46Issue(9)：783-786,4.DOI:10.12007/j.issn.0258-4646.2017.09.004
雷帕霉素对老龄帕金森病模型小鼠线粒体损伤的改善作用
Protective Effect of Rapamycin against Mitochondrial Injury in an Aging Parkinson's Disease Mouse Model
摘要
Abstract
Objective To investigate the effect of rapamycin (RAPA) on mitochondrial injury in a mouse model of aging Parkinson's disease (PD).Methods Forty senescence-accelerated prone mice 8 (SAMP8) (12-month old) were randomly divided into 5 groups:blank group,model group,and RAPA low-,middle-,and high-dose groups.Mice in the model group and three RAPA groups were administered a subcutaneous injection of MPTP to generate the PD model.RAPA at 1,2,and 4 mg· kg 1· d 1 was administered from 7 days before,5 days during,and 7 days after the PD model preparation to the RAPA groups;an equal volume of sterile saline was administered to the other two groups.After the administration,behavioral test scores,dopamine levels,transmembrane potential of mitochondria,and activity of mitochondrial complex Ⅰ in the 5 groups were evaluated.Results Behavioral scores,dopamine levels,transmembrane potential,mitochondrial complex Ⅰ activity of mice in the model group were significantly decreased compared with the blank group (P ＜ 0.05 respectively).All indexes in the RAPA groups were significantly improved compared to the model group (P ＜ 0.05 respectively).There was no significant difference among the three RAPA groups.Conclusion RAPA has a protective effect on aging PD model mice,and its mechanism may be related to the protection against mitochondrial damage.关键词
雷帕霉素/老龄/帕金森病/线粒体Key words
rapamycin/aging/Parkinson's disease/mitochondria分类
医药卫生引用本文复制引用
张忠霞,马晓伟,王彦永,邱会卿,王铭维..雷帕霉素对老龄帕金森病模型小鼠线粒体损伤的改善作用[J].中国医科大学学报,2017,46(9):783-786,4.基金项目
河北省医学科学研究重点课题计划(20160202) （20160202）
河北医科大学第一医院“星火”青年科研项目(XH201711) （XH201711）
