中国药理学通报2017，Vol.33Issue(12)：1655-1660,6.DOI:10.3969/j.issn.1001-1978.2017.12.007
北美海篷子三萜皂苷bigelovii E抑制mTOR信号通路诱导乳腺癌MCF-7细胞凋亡
Bigelovii E,a triterpenoid compound of Salicornia bigelovii,induced apoptosis of breast cancer MCF-7 cells via inhibiting mTOR / P70S6K / 4-EBP signaling
摘要
Abstract
Aim To demonstrate that bigelovii E,one of the triterpenoid compounds isolated from Salicornia bigelovii Torr. ,induced apoptosis and inhibited prolif-eration of human breast cancer cells MCF-7. Methods MTT and Clonogenic were used to detect the anti-proliferative effect of bigelovii E on human tumor cells. Hoechst 33258 staining was used to observe the chan-ges of cell morphology in the treatment with bigelovii E. The effect of bigelovii E on cell apoptosis,cell mi-tochondrial membrane potential and the ROS level was analyzed by flow cytometry. The expression of mito-chondrial apoptotic pathway and its regulated proteins were analyzed by Western blot. Results Bigelovii E was most sensitive to MCF-7;bigelovii E had lower toxicity to normal cells HLF1;bigelovii E inhibited the colony formation of breast cancer MCF-7;the apoptosis pattern induced by bigelovii E was detected by Hoechst 33258;Western blot showed that bigelovii E could down-regulate the phosphorylation of mTOR,p70S6K and 4-EBP,up-regulate the expression of Bcl-xl and Mcl-1 proteins,promote the mitochondrial apoptosis pathway,reduce the mitochondrial membrane poten-tial,and induce ROS,leading to mitochondrial func-tion damage,and ultimately inducing apoptosis. Con-clusion Bigelovii E regulates mitochondrial apoptosis pathway through mTOR pathway,induces apoptosis and has a good anti-tumor effect.关键词
北美海篷子/bigeloviiE/乳腺癌/细胞凋亡/mTOR/抗肿瘤Key words
Salicornia bigelovii/bigelovii E/breast cancer/apoptosis/mTOR/anti-tumor分类
医药卫生引用本文复制引用
黄真真,张丹玉,师琪,管福琴,王奇志,王鸣,冯煦,单宇..北美海篷子三萜皂苷bigelovii E抑制mTOR信号通路诱导乳腺癌MCF-7细胞凋亡[J].中国药理学通报,2017,33(12):1655-1660,6.基金项目
国家自然科学基金资助项目(No 81402829) （No 81402829）
江苏省科技计划项目(No BM2015019) （No BM2015019）
