中国药理学通报2017，Vol.33Issue(12)：1673-1678,6.DOI:10.3969/j.issn.1001-1978.2017.12.010
蛋白酪氨酸磷酸酶Shp2调节香烟提取物诱导的肺上皮间质转化
Shp2 regulates cigarette smoke extract-induced epithelial-mesenchymal transition
摘要
Abstract
Aim To explore the effects of Shp2 on cig-arette smoke extract (CSE)-induced epithelial-mesen-chymal transition (EMT). Methods The effects of CSE on TGF-β1 levels in epithelial cells were meas-ured by Q-PCR and ELISA. Immunofluorescent stai-ning was used to assess the expressions of CSE-induced EMT-related markers. The activation of CSE-induced Shp2,Smad2 was investigated by Western blot. Re-sults CSE induced Shp2 phosphorylation in a concen-tration-dependent manner in A549 cells. PHPS1 inhib-ited the increase in mRNA and protein expression of TGF-β1 induced by CSE. PHPS1 regulated the expres-sions of CSE-induced EMT markers (down-regulation of E-cadherin,up-regulation expression of Vimentin and α-SMA). The inhibition of either Shp2 inhibitor or Shp2 siRNA decreased Smad2 phosphorylation induced by CSE. Conclusions CSE initiates EMT through the Shp2 / Smad2 signaling pathway,which is activated by CSE through TGF-β1 generation. It is suggested that Shp2 might be a possible new target for COPD and lung cancer therapy.关键词
上皮间质转化/转化生长因子β1/香烟提取物/蛋白酪氨酸磷酸酶Shp2/慢性阻塞性肺病/肺癌Key words
epithelial-mesenchymal transition/TGF-β1/cigarette smoke extract/Shp2/COPD/lung canc-er分类
医药卫生引用本文复制引用
沈辉娟,蒋俊霞,李芬芬,谢强敏,颜小锋..蛋白酪氨酸磷酸酶Shp2调节香烟提取物诱导的肺上皮间质转化[J].中国药理学通报,2017,33(12):1673-1678,6.基金项目
国家自然科学基金资助项目(No 30973542) （No 30973542）
