中国药理学通报2017，Vol.33Issue(12)：1691-1697,7.DOI:10.3969/j.issn.1001-1978.2017.12.013
梓醇对大脑皮质神经元的抗衰老保护作用研究
Study of anti-aging protective effect of catalpol on cultured cortical neurons from rats
摘要
Abstract
Aim To investigate the anti-aging effect of catalpol on normal cortical neurons and its possible mechanism. Methods Primary cultured cortical neu-rons from 24 h newly born rats were dissociated and cultured. The cells were divided into normal group, catalpol (0. 1 mg · L - 1 )group,catalpol (1 mg · L - 1 )group and catalpol (10 mg·L - 1 )group. Neu-rons were cultured for 13d,and the cell morphology was observed by microscope. Cell activity was detected by MTT assay. p-S6 and Map-2 of 13d neurons were detected by immunofluorescence to reveal cell viability and the axon growth. GAP-43,p-S6,PI3K,p-PI3K, Akt,p-Akt,mTOR and p-mTOR protein expression of 13d neurons were detected by Western blot. Results Compared with normal group,different concentrations of catalpol could delay the aging of neurons,promote their survival and increase cell viability. The nμmber of p-S6 positive cells increased. Different concentra-tions of catalpol could promote axon growth. GAP-43, p-S6,PI3K,p-PI3K,Akt,p-Akt,mTOR and p-mTOR protein expression of 13d neurons increased. Conclusions Catalpol has anti-aging effects on cere-bral cortical neurons,and its mechanism may be relat-ed to regulating PI3K/ Akt/ mTOR signaling pathway and enhancing neuronal growth activity.关键词
梓醇/皮质神经元/抗衰老/细胞活性/轴突生长/PI3K/Akt/mTORKey words
catalpol/cortical neurons/anti-aging/cell viability/axon growth/PI3K/ Akt/ mTOR分类
医药卫生引用本文复制引用
王静欢,刘珂,万东,祝慧凤..梓醇对大脑皮质神经元的抗衰老保护作用研究[J].中国药理学通报,2017,33(12):1691-1697,7.基金项目
国家自然科学基金资助项目(No 81073084) （No 81073084）
重庆市自然科学基金资助项目(No CSTC2010BB5127,CSTC2014jcyj A10083) （No CSTC2010BB5127,CSTC2014jcyj A10083）
教育部中央高校重点项目(No XDJK2012B010) （No XDJK2012B010）
