中国药理学通报2017，Vol.33Issue(12)：1707-1712,6.DOI:10.3969/j.issn.1001-1978.2017.12.016
吴茱萸次碱通过抑制TLR4/NF-κB信号通路保护大鼠心肌缺血/再灌注损伤
Rutaecarpine protects against myocardial ischemia / reperfusion injury through inhibiting Toll-like receptor 4 / NF-κB signaling pathway in rats
摘要
Abstract
Aim To investigate whether the protection of rutaecarpine against myocardial ischemia / reperfusion (I/ R ) injury is mediated by Toll-like receptor 4 (TLR4)/ NF-κB pathway,and whether these effects are related to the release of calcitonin gene-related peptide(CGRP)in plasma. Methods Sprague-Daw-ley rats were subjected to 60 min of ligation of the left anterior descending coronary followed by 3h of reperfu-sion to induce I/ R injury. Rats were pretreated with rutaecarpine 10 min before the ligation,and some rats were pretreated with capsazepine 2 min before rutae-carpine administration. Myocardial infarct size,CGRP concentration in plasma and creatine kinase (CK)ac-tivity in serum were measured. The expression of TLR4 mRNA in myocardial tissue was determined by RT-PCR. The protein expression of TLR4 and NF-κB in myocardial tissue was analyzed by immunohistochemis-try. Results Rutaecarpine (100,300 μg · kg - 1 , iv)significantly reduced the infarct size and the serum CK activity concomitantly with the increase in plasma CGRP concentration (P < 0. 05). Importantly,the ex-pression of TLR4 (both mRNA and protein)and NF-κB (protein)was increased by myocardial I/ R injury, and dramatically inhibited by rutaecarpine pretreatment (P < 0. 05). All these effects of rutaecarpine were a-bolished by capsazepine (1. 5 mg·kg - 1 ,iv),a spe-cific antagonist for vanilloid receptor-1. Conclusion Rutaecarpine protects against myocardial I/ R injury by inhibiting TLR4 / NF-κB pathway,which is related to the increased release of CGRP.关键词
Toll样受体4/降钙素基因相关肽/缺血/再灌注损伤/吴茱萸次碱/心脏/大鼠Key words
Toll-like receptor 4/calcitonin gene-re-lated peptide/ischemia / reperfusion injury/rutae-carpine/heart/rats分类
医药卫生引用本文复制引用
杨晶,张晓坚,胡长平..吴茱萸次碱通过抑制TLR4/NF-κB信号通路保护大鼠心肌缺血/再灌注损伤[J].中国药理学通报,2017,33(12):1707-1712,6.基金项目
河南省医学科技攻关计划省部共建项目 (No 201601001) （No 201601001）
河南省医学科技攻关计划项目 (No 201403019) （No 201403019）
