中国药理学通报2017，Vol.33Issue(12)：1729-1735,7.DOI:10.3969/j.issn.1001-1978.2017.12.020
对羟基苯甲醛对脑缺血/再灌注损伤的保护作用研究
The protective effects of 4-hydroxybenzaldehyde on cerebral ischemia / reperfusion injury
摘要
Abstract
Aim To verify the mechanisms underlying the protective effects of the 4-hydroxybenzaldehyde (4-HBd)on cerebral ischemia / reperfusion in vivo and in vitro. Methods The model of focal cerebral ischemi-a / reperfusion in rats was established by the suture-oc-clusion method. After reperfusion,neurological score and cerebral infarction rate were assessed. Meanwhile, after pretreatment with 4-HBd for 24 h,the primary cultured cortical neuron cells were exposed to oxygen glucose deprivation / reoxygenation (OGD/ Rep). The survival rate, MDA, SOD, NO, Bax, Bcl-2 and caspase-3 concentrations were assessed. Results Pre-treatment with 4-HBd significantly decreased neurologi-cal score (P < 0. 05)and cerebral infarction rate (P <0. 01)in the cerebrum after MCAO/ R injury. Further-more,4-HBd significantly increased cell survival rate (P < 0. 05),decreased MDA content (P < 0. 05),in-creased SOD activity (P < 0. 05),decreased NO levels (P < 0. 05),decreased caspase-3 (P < 0. 05),in-creased Bcl-2 (P < 0. 05)but decreased Bax (P <0. 05)levels and therefore increased Bcl-2 / Bax ratio in the cerebrum after OGD/ R injury. Conclusions 4-HBd has significant protective effect on MCAO/ R rats, and the mechanism may be related to decreasing NO levels,Bax,Bcl-2,caspase-3 and reducing neuronal apoptosis.关键词
对羟基苯甲醛/天麻/脑缺血/再灌注损伤/连二亚硫酸钠/缺糖缺氧/复糖复氧/原代皮层神经元/细胞凋亡Key words
4-hydroxybenzaldehyde/Gastrodia elata Blume/cerebral ischemia / reperfusion/Na 2 S2 O4/oxy-gen-glucose deprivation / reintroduction/primary corti-cal neurons/apoptosis分类
医药卫生引用本文复制引用
周晓楠,宋雪兰,李艳,宋波,杨建光,何芳雁..对羟基苯甲醛对脑缺血/再灌注损伤的保护作用研究[J].中国药理学通报,2017,33(12):1729-1735,7.基金项目
国家自然科学基金资助项目(No 81560664) （No 81560664）
云南省应用基础研究项目(No 2016FA035) （No 2016FA035）
云南省教育厅资助项目(No 2017ZZX287) （No 2017ZZX287）
