中国药理学通报2017，Vol.33Issue(12)：1740-1743,4.DOI:10.3969/j.issn.1001-1978.2017.12.022
过表达THEM4对高糖诱导的人肾小管上皮细胞胶原分泌的影响
Role of up-regulation of THEM4 on collagen secretion in human renal tubular epithelial cells treated with high glucose
摘要
Abstract
Aim To investigate the effect of thioester-ase superfamily member 4(THEM4)expression on col-lagen secretion in human renal proximal tubular epithe-lial cells (HKC)treated with high glucose. Methods In order to examine the direct effect of THEM4 ex-pression vector on PI3K/ Akt pathway and collagen se-cretion,pYr-ads-4-THEM4 expression vector was con-structed and transfected into the HKC with lipo-fectamine 2000 in vitro. HKC cells were randomly di-vided into four groups:normal glucose group (Con-trol),high glucose group (HG),high glucose plus pYr-ads-4-THEM4 vector group (HG + THEM4 vec-tor) and high glucose plus pYr-adshuttle-4 vector group (HG + V vector). After 48 h with HG stimula-tion,the cells were collected for extraction of protein and phospho-Akt (Ser 473),THEM4,TGF-β1 andα-SMA protein expression were examined by Western blot and immunofluorescence staining respectively. Col Ⅰ and Col Ⅲ were detected using the competitive sandwich ELISA kit according to the manufacturer's instructions. Results High glucose inhibited THEM4 expression,and induced increased phospho-Akt (Ser 473),TGF-β1,α-SMA and secreted ColⅠand secre-ted Col Ⅲ in HKC cells. Up-regulation of THEM4 re-versed high glucose-induced decreased THEM4,in-creased phospho-Akt (Ser 473),TGF-β1,α-SMA, secreted Col Ⅰ and secreted Col Ⅲ in HKC cells. Conclusion The up-regulation of THEM4 may de-crease Col Ⅰ and Col Ⅲ secretion by inhibiting the phosphorylation of Akt and down-regulating the expres-sion of TGF-β1 and α-SMA in high glucose-induced HKC cells.关键词
硫酯酶超家族成员4/PI3K/Akt/高糖/人肾小管上皮细胞/转化生长因子β1/Ⅰ型胶原/Ⅲ型胶原Key words
thioesterase superfamily member 4/PI3K/ Akt/high glucose/human renal tubular cells/TGF-β1/collagen Ⅰ/collagen Ⅲ分类
医药卫生引用本文复制引用
陈宁,郝军,朱桂云,安晓颖,康丽菲,李晓霞,杨永辉..过表达THEM4对高糖诱导的人肾小管上皮细胞胶原分泌的影响[J].中国药理学通报,2017,33(12):1740-1743,4.基金项目
河北省医学科学研究重点课题计划(No 20150604) （No 20150604）
