中国药理学通报2017，Vol.33Issue(12)：1749-1753,5.DOI:10.3969/j.issn.1001-1978.2017.12.024
靶向ESCCAL_1基因的siRNA纳米复合物的制备及体外对食管癌EC-9706细胞的抑制作用
Preparation and in vitro study of ESCCAL_1-targeted siRNA gene delivery of nanocomposite for treating esophageal cancer EC-9706
摘要
Abstract
Aim To investigate the influence of inhibi-tory nanocomposite on EC-9706 cells and the effect of nanocomposite on ESCCAL _ 1 LncRNA expression, siRNA-loaded nanocomposite being prepared as non-vi-rus delivery system Methods Mesoporous silica nano-particles were prepared by sol-gel method under room temperature and coated by cationic polymerpolyethylen-imine (PEI)on the surface to stay positive charge, which could facilitate its combination with negatively charged ESCCAL _ 1 siRNA. The size and surface charge of nanocomposite were determined by laser par-ticle analyzer and TEM. The inhibitory rate of nanopar-ticles on EC-9706 cells was detected by MTT methods. Entrapment efficiency was determined by agarose gel e-lectrophoresis. The uptake-siRNA was detected by flu-orescence microscope. The expression of ESCCAL _1 LncRNA was detected by RT-PCR. Results The MSNP appeared to have a high dispensability and hom-ogeneous size by particle size analyzer and transmission electron microscopy(TEM). The formed nanoparticles had a surface mesoporous diameter of 3 ~ 5 nm. The proliferation of ESCCAL_1 was inhibited significantlly (P < 0. 05),and the 72h inhibitory rate was (54. 93 ± 2. 6)%;the siRNA loading could be effectively up-taken by EC-9706 cells;ESCCAL_1 silencing efficien-cy was 69. 5% . Conclusions The tumor targeting nanocomposite with high encapsulation efficiency is prepared. The proliferation of esophageal cancer EC-9706 cells can be effectively inhibited by anocompos-ite-mediated siRNA,and the expression of ESCCAL_1 is effectively silenced in EC-9706 cells. The nanocom-posite is an efficient gene delivery system and may have potential application in gene therapy.关键词
食管鳞状细胞癌特异相关LncRNA转录物1/siR-NA/肿瘤靶向性/基因治疗/食管癌/EC-9706细胞Key words
ESCCAL _ 1/siRNA/tumor targeting/gene therapy/esophageal cancer/EC-9706 cells分类
医药卫生引用本文复制引用
韩鹏黎,孙蕾,吕朋举,龚芬芬,夏天,曹巍..靶向ESCCAL_1基因的siRNA纳米复合物的制备及体外对食管癌EC-9706细胞的抑制作用[J].中国药理学通报,2017,33(12):1749-1753,5.基金项目
国家自然科学基金资助项目(No 31570899) （No 31570899）
河南省医学科技攻关计划项目(No 201403273) （No 201403273）
