中国药理学通报2017，Vol.33Issue(12)：1766-1770,5.DOI:10.3969/j.issn.1001-1978.2017.12.027
基于1 H-NMR护肝片抗大鼠急性肝损伤的代谢组学研究
Metabonomic analysis of Hugan Tablets on CCl4-induced acute hepatic injury in rats based on 1 H-NMR
摘要
Abstract
Aim To identify the potential biomarkers associated with carbon tetrachloride(CCl 4 )-induced a-cute hepatic injury in rats and explore the therapeutic effect of Hugan Tablets(HGT). Methods The model was established by intraperitoneal injection of CCl4 in oil(1 : 1,V/ V)with a dosage of 1 mL·kg - 1 body weight to rats once. The levels of aspartate aminotrans-ferase(AST),alanine aminotransferase(ALT),alka-line phosphatase (ALP ) and lactate dehydrogenase (LDH)in serum of rats were determined. Moreover,a proton nuclear magnetic resonance (1 H-NMR)based metabonomic approach in combination with multivariate data analysis was applied to demonstrate CCl4-induced acute hepatic injury metabolic perturbations in rat urine and feces and identify the corresponding metabolic bio-markers. The intervention effect of HGT was evaluated based on the changes of metabolic phenotype and po-tential biomarkers related to acute hepatic injury. Re-sults The levels of AST,ALT,ALP and LDH in ser-um of rats with acute hepatic injury were significantly reduced by administration of HGT,respectively. The disturbed metabolic state associated with CCl4-induced acute hepatic injury in rat urine and feces could be re-stored by HGT. Meanwhile,five potential biomarkers (2-oxoglutarate,citrate,creatinine,trimethylamine N-oxide,hippurate)in rat urine and three potential bio-markers(butyrate,glucose,uracil)in rat feces related to acute hepatic injury were reversed by administration of HGT,respectively. Conclusion HGT exerts pro-tective effects against CCl4-induced acute hepatic inju-ry in rats,which is probably mediated by regulation of tricarboxylic acid cycle and gut microbiota metabolism.关键词
护肝片/四氯化碳/肝损伤/代谢组学/核磁共振/生物标志物Key words
Hugan Tablets/carbon tetrachloride/a-cute hepatic injury/metabonomics/NMR/biomarkers分类
医药卫生引用本文复制引用
龚梦鹃,巫圣乾,岳贺,王淑美,梁生旺,邹忠杰..基于1 H-NMR护肝片抗大鼠急性肝损伤的代谢组学研究[J].中国药理学通报,2017,33(12):1766-1770,5.基金项目
国家自然科学基金资助项目(No 81603397,81403075) （No 81603397,81403075）
