中国药业2017，Vol.26Issue(22)：1-3,3.DOI:10.3969/j.issn.1006-4931.2017.22.001
鸢尾甲黄素A对脂多糖诱导小鼠RAW264.7细胞分泌 炎性因子的调节作用
Regulation Effect of Iristectorigenin A on Secretion of Inflammatory Factor in Mouse RAW264. 7 Cells Induced by Lipopolysaccharide
摘要
Abstract
Objective To investigate the regulation effect of iristectorigenin A on secretion of inflammatory factors (NO and IL-6)in mouse RAW264. 7 macrophage cells induced by lipopolysaccharide(LPS). Methods LPS was adopted to induce RAW264. 7 macrophage cells in order to establish the model of cellular inflammatory response. Different concentrations of iristectorigenin A were used to inter-vene the RAW264. 7 macrophage cells,and the blank control was set. The effects of different concentrations of iristectorigenin A on the viability of RAW264. 7 cells were determined by MTT method,the production of NO was detected by Griess method,and the content of inflammatory factor(IL-6)in cell supernatant was detected by ELISA. Results Iristectorigenin A can significantly inhibit the produc-tion of NO and IL-6 in RAW264. 7 cells induced by LPS, and the concentration of the action was in a concentration dependent manner. Conclusion The anti-inflammatory effect of iristectorigenin A may be related to the reduction of the production of inflamma-tory factors(NO and IL-6).关键词
鸢尾甲黄素A/脂多糖/RAW264.7细胞/一氧化氮/白细胞介素6/小鼠Key words
iristectorigenin A/lipopolysaccharide/RAW264. 7 cell/NO/IL-6/mouse分类
医药卫生引用本文复制引用
邹桂欣,孙小玲,王光函,尤献民,李国信..鸢尾甲黄素A对脂多糖诱导小鼠RAW264.7细胞分泌 炎性因子的调节作用[J].中国药业,2017,26(22):1-3,3.基金项目
国家自然科学基金[81273927] （）
国家"重大新药创制"项目[SQ2017ZX090328]. （）
