中国药业2017，Vol.26Issue(22)：28-30,3.DOI:10.3969/j.issn.1006-4931.2017.22.009
高效液相色谱法测定左旋甲状腺素钠片的含量及溶出度
Content and Dissolution Determination of Levothyroxine Sodium Tablets by HPLC
常海莹1
作者信息
- 1. 河南省新乡市食品药品检验所,河南新乡 453000
- 折叠
摘要
Abstract
Objective To establish an HPLC method for content and dissolution determination of Levothyroxine Sodium Tablets. Methods The content of levothyroxine sodium in two kinds of sodium tablets was determined by HPLC,and the dissolution of two kinds of Levothyroxine Sodium Tablets was determined according to the determination method of Chinese Pharmacopoeia. The chromatographic column was Hypersil BDS C18 column(150 mm × 4. 6 mm,5 μm),the column temperature was 25 ℃,the mobile phase consisted of methanol - water - phospho-ric acid (600 :400 :1),the detection wavelength was 225 nm,and the flow rate was 1. 5 mL/min. Results The percentages of the contents of the two kinds of Levothyroxine Sodium Tablets were 101. 39％ and 99. 32％,respectively. The time and m value of 50％ and 63. 2％ dis-solution of two tablets showed significant difference (P < 0. 05). Conclusion HPLC is the effective method for the determination of Levothy-roxine Sodium Tablets. There is no significant difference in the content of two kinds of Levothyroxine Sodium Tablets,but the dissolution rate is obviously different.关键词
左旋甲状腺素钠/高效液相色谱法/含量/溶出度Key words
levothyroxine sodium/HPLC/content/dissolution分类
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常海莹..高效液相色谱法测定左旋甲状腺素钠片的含量及溶出度[J].中国药业,2017,26(22):28-30,3.
