中国中医药信息杂志2017，Vol.24Issue(12)：34-37,4.DOI:10.3969/j.issn.1005-5304.2017.12.009
四神丸对脾肾阳虚泄泻大鼠胃窦组织Ghrelin-CaM-MLCK信号通路的影响
Effects of Sishen Pills on Ghrelin-CaM-MLCK Signaling Pathway in Sinuses Ventriculi of Rats with Diarrhea of Yang Deficieney of Spleen and Kidney
摘要
Abstract
Objective To explore the effects of Sishen Pills on Ghrelin-CaM-MLCK signaling pathway with sinuses ventriculi in diarrhea rats of yang deficieney of spleen and kidney; To discuss its mechanism of action. Methods A model rats with diarrhea model of yang deficieney of spleen and kidney was established by treated with intragastric administration of adenine and Senna water decoction to replicate. 72 SD rats were randomly divided into normal group, model group, positive medicine group, Sishen Pills high-, medium- and low-dose groups, with 12 rats in each group. Each medication group was given relevant medicine for gavage, for 14 days. Immunohistochemistry and Western blot were used to detect the expressions of Ghrelin and GHSR, CaM, and MLCK protein in the sinuses ventriculi. Results In the model group, Ghrelin, GHSR, CaM and MLCK protein expressions of the sinuses ventriculi significantly increased (P<0.01). In each medication group, protein expression of the indexes was lower than the model group (P<0.05, P<0.01). The Sishen Pills high-dose group was particularly obvious. Conclusion The effect of Sishen Pills on diarrhea model rats of yang deficieney of spleen and kidney may be associated with regulation of index protein expression in Ghrelin-CaM-MLCK signaling pathway.关键词
四神丸/泄泻/胃促生长素/胃促生长素受体/钙调素/肌球蛋白轻链激酶/大鼠Key words
Sishen Pills/diarrhea/Ghrelin/GHSR/CaM/MLCK/rats分类
医药卫生引用本文复制引用
蔺晓源,邓娜,蔡莹,刘芳..四神丸对脾肾阳虚泄泻大鼠胃窦组织Ghrelin-CaM-MLCK信号通路的影响[J].中国中医药信息杂志,2017,24(12):34-37,4.基金项目
国家自然科学基金(81403380) （81403380）
湖南省教育厅科研项目(15B178、17C1636) （15B178、17C1636）
