中国中医药信息杂志2017，Vol.24Issue(12)：47-51,5.DOI:10.3969/j.issn.1005-5304.2017.12.012
黄芪多糖对X线辐射骨髓间充质干细胞向成骨方向分化的影响
Effects of Astragalus Polysaccharide on Osteoblastic Differentiation of Bone Mesenchymal Stem Cells Induced by X-ray Radiation
摘要
Abstract
Objective To investigate the effects of astragalus polysaccharide (APS) on bone mesenchymal stem cells (BMSCs) to osteoblasts differentiation induced by X-rays; To discuss its mechanism of action. Methods CCK-8 method was used to select different concentrations of APS for the proliferation ability of BMSCs with 2 Gy X-ray radiation, and the best concentration was determined. Cells were divided into blank group, APS group, radiation group, radiation+APS group. APS group and radiation+APS group were given the best concentration of APS for 3 days, radiation group and radiation+APS group were given 2 Gy X-ray radiation. After radiation, 2 mL osteogenesis induced liquid was added in each group, every 3 day. After 15 day''s induction, inverting microscope was used to observe morphology, and alizarin red staining to detect the area of the calcium nodules in each group. Western blot was used to detect the specific marker protein osteopontin and osteocalcin expression of each group. Results Compared with the blank group, the proliferation ability of radiation group was obviously lower (P<0.05); compared with radiation group, the proliferation ability of radiation+APS significantly increased (P<0.05); the strongest promoting proliferation of APS was 50μg/mL, therefore, it was selected as the best concentration. In terms of morphology, inverted microscope showed that secretion of crystals of radiation group was obviously reduced compared with the blank group and APS group, and secretion of crystals of radiation+APS group was significantly elevated compared with radiation group. In osteogenesis ability, compared with the blank group, the cell calcium nodule area of APS group had a certain reduce, but the radiation group had a significantly reduced (P<0.05). Compared with the radiation group, the cell calcium nodule area of radiation+APS group obviously increased (P<0.05). In terms of osteogenesis specific marker protein expression, compared with the blank group, the expression of osteopontin of APS group was slightly declined, and the expression of osteocalcin was slightly elevated, but the expression of osteopontin and osteocalcin of radiation group was significantly lowered (P<0.05). Compared with the radiation group, the expression of osteopontin and osteocalcin of radiation+APS group was significantly higher (P<0.05). Conclusion APS has protective effects on osteoblastic differentiation ablility of BMSCs induced by X-ray radiation.关键词
黄芪多糖/X线辐射/骨髓间充质干细胞/成骨分化Key words
astragalus polysaccharide/X-ray radiation/BMSCs/osteoblasts differentiation分类
医药卫生引用本文复制引用
王磊,卢志伟,许小敏,刘永琦,张利英,张苡铭,张丽昕,何进鹏,丁楠..黄芪多糖对X线辐射骨髓间充质干细胞向成骨方向分化的影响[J].中国中医药信息杂志,2017,24(12):47-51,5.基金项目
国家自然科学基金(81473457) （81473457）
