浙江农林大学学报2017，Vol.34Issue(6)：1038-1043,6.DOI:10.11833/j.issn.2095-0756.2017.06.010
吲哚丁酸对核桃嫩枝扦插生根及内源激素变化的影响
Adventitious root formation with IBA and endogenous hormones dynamics in walnut soft-cuttings
摘要
Abstract
Phytohormones are an important factor in regulating adventitious root formation. To provide a refer-ence for discussing the role of phytohormones on adventitious root formation in walnut, the effect of exogenous hormones on endogenous hormones was analyzed by measuring the level of endogenous hormones during the cutting process of a rejuvenated softwood, Juglans (walnut) 'Zhongningsheng'. The rejuvenated softwood was first induced by exogenous indole-3-butyric acid (IBA) and then, using High Performance Liquid Chromatogra-phy-Mass Spectrometry (HPLC-MS), the endogenous hormones:Indole-3-acetic acid (IAA), Gibberellic acid (GA3), Abscisic Acid (ABA), and Zeatin (ZT); basal segments of soft-cuttings were measured followed by measurement of the rooting situation, student's test were used for the treatment of the data. Results showed strong differences between samples induced and not induced by IBA. The rooting rate of rejuvenated softwood induced by IBA was up to 98.1%; whereas, in the control the rooting rate was 0 and the mortality rate was 75.5%. The rooting process experienced several stages, such as thickening of the cambium, meristem cell for-mation, root primordia initial cell formation, root primordia formation, and root development through observation of the anatomic structure. Additionally, the external morphology showed thickening and cracking. During soft-wood cutting, endogenous hormone levels in rejuvenated soft shoots induced by IBA changed noticeably. Also, IAA increased first and reached its peak at day 5, then decreased as did GA3. However, ABA decreased first and then increased. In the early stages, ZT changed little but did increase after root primordia induction. The level of IAA in softwood induced by IBA improved; however, the effects on ZT were small. So the changes of endogenous hormones induced by IBA promoted adventitious root formation.关键词
植物学/核桃/嫩枝扦插/不定根/高效液相色谱串联质谱法/植物激素Key words
botany/walnut/softwood cutting/adventitious root/HPLC-MS/phytohormones分类
农业科技引用本文复制引用
刘昊,宋晓波,周乃富,马庆国,裴东..吲哚丁酸对核桃嫩枝扦插生根及内源激素变化的影响[J].浙江农林大学学报,2017,34(6):1038-1043,6.基金项目
国家林业公益性行业科研专项(201304712) （201304712）
国家自然科学基金资助项目(31672126) （31672126）
