浙江农林大学学报2017，Vol.34Issue(6)：1065-1070,6.DOI:10.11833/j.issn.2095-0756.2017.06.014
浙东白鹅骨骼肌发育相关因子MRFs,Pax3和Pax7基因的表达规律
Expression patterns of MRFs, Pax3, and Pax7 during skeletal muscle development in Zhedong white geese
摘要
Abstract
The family of myogenic regulatory factors (MRFs) played an important role in the process of skeletal muscle differentiation during the embryonic stage and the early stage of hatching with Pax3 and Pax7, two transcription factors of the PAX family, guiding the formation of various processes during the growth and devel-opment of muscle fiber. To determine the expression profiles of these genes during the embryonic and muscle development stages in geese, the expression pattern of MRFs, Pax3, and Pax7 during the embryonic period (7th, 11th, 15th, 19th, 23th, 27th day in embryonic (E) age) and early birth (7-day-age) in Zhedong White Geese was studied. Analysis included extracting RNA and synthetic DNA along with real-time PCR followed by further analysis on their relationship with the development of breast and leg muscles. We took three samples for tissue of each period, and the significance of expression was analyzed with SASS. Results revealed that MRFs, Pax3, and Pax7, were all related to the development of skeletal muscles in geese with different expression pat-terns. MRFs and Pax7 showed peak expression during the period from E11 to E15, and the highest expression of Pax3 with a declining expression appeared at E7. Findings suggested that the expression patterns of these genes were highly matched with their function during muscle development and provided a theoretical basis for further investigation of the regulatory mechanism of MRFs, Pax3, and Pax7 during skeletal muscle development in geese.关键词
动物学/鹅/骨骼肌发育/MRFs/Pax3/Pax7Key words
zoology/goose/skeletal muscle development/myogenic regulatory factors (MRFs)/Pax3/Pax7分类
农业科技引用本文复制引用
王吟方亭,任婷,朱松会,梁世日,何珂,赵阿勇..浙东白鹅骨骼肌发育相关因子MRFs,Pax3和Pax7基因的表达规律[J].浙江农林大学学报,2017,34(6):1065-1070,6.基金项目
国家自然科学基金资助项目(31372349) （31372349）
浙江省自然科学基金资助项目(LQ14C030002) （LQ14C030002）
