质谱学报2017，Vol.38Issue(6)：655-663,9.DOI:10.7538/zpxb.2016.0148
QuEChERS-GPC-GC/MS同时测定鱼肉中9种羟基类兽药残留
Determination of 9 Hydroxy Veterinary Drug Residues in Fish by QuEChERS-GPC-GC/MS
摘要
Abstract
A method of QuEChERS-online gel permeation chromatography gas chroma-tography-mass spectrometry (QuEChERS-GPC-GC/MS) was established for 9 hydroxy veterinary drug residues in fish .GPC system was adopted for online purifying pretreat-ment to remove most of the fat in fish so as to effectively reduce the time of sample pretreatment .At the same time ,the selective ion monitoring acquisition mode could greatly reduce the interference and provide selectivity .Extracted with acetonitrile and purified by dispersive solid phase extraction method , the analytes were detected and determined under SIM scan type with tandem mass analyzer .The analytical method was well validated ,and the results were obtained at the respect of precision ,repeatability and spiked recovery .The detection limits of 9 hydroxy veterinary drug residues are 0.3-1.0 μg/kg with the linear range of 0.5-20.0 μg/L .The overall recoveries are between 63.5% and 90.2% with the relative standard deviations (RSDs ) of 3.6%-15.4% . Finally ,the validated method was carried out in total of 10 samples to evaluate 9 hydroxy veterinary drug residues ,and one sample has chloramphenicol positive ,and the content is 6.1 μg/kg .This method is suitable for the determination of 9 hydroxy veteri-nary drugs in fish .关键词
羟基类兽药/QuEChERS/在线凝胶渗透色谱-气相色谱-质谱联用法(GPC-GC-MS)/鱼肉Key words
hydroxy veterinary drug/QuEChERS/gel permeation chromatography gas chromatography-mass spectrometry (GPC-GC/MS)/fish分类
化学化工引用本文复制引用
薛良辰,蔡杰,黄晶,蔡勤仁,郑璇,刘陆,凌芸辉,李忠,彭玉芬,陈敬,蔡德琳..QuEChERS-GPC-GC/MS同时测定鱼肉中9种羟基类兽药残留[J].质谱学报,2017,38(6):655-663,9.基金项目
2014年珠海市本级农业专项资金项目(2014ZH26) （2014ZH26）
2014 年珠海出入境检验检疫局科技计划项目(ZH 2013-5)资助 （ZH 2013-5）
