质谱学报2017，Vol.38Issue(6)：678-689,12.DOI:10.7538/zpxb.2016.0182
基于UPLC-Triple TOF MS/MS技术分析不同产地何首乌的差异化学成分
Difference of Chemical Compositions in Polygoni Multifori Radix from Different Habitats by UPLC-Triple TOF MS/MS
摘要
Abstract
In order to study the influence of the different origin on the synthesis and accumulation of metabolites in Polygoni Multifori Radix ,the UPLC-Triple TOF MS/MS method combined with multivariate statistical analysis was used to analyze the differences of chemical compositions in Polygoni Multifori Radix from different habi-tats .Through the analysis of the multistage tandem mass spectrometry ,the characteris-tic peaks were extracted with mass spectrometry data peak matching ,peak alignment and noise filtering .Principal component analysis (PCA ) and partial least-squares dis-criminant analysis (PLS-DA) were used for data processing .The chemical compositions were identified or tentative presumed according to MS accurate mass and MS/MS spec-trometry fragmentation information ,combined with the software of database search and literature .The results showed that the differences among samples of Polygoni Multifo-ri Radix from different habitats are distinguishable .A total of 36 differential chemical compositions were screened in Polygoni Multifori Radix from different habitats ,and 33 chemical constituents of which were identified .Among of them ,there were 12 kinds of common differential chemical compositions , such as 3 ,5-dihydroxyphenol-1-O-β-D-(6″-O-galloyl)-glucopyranoside ,gallic acid ,pocyanidin B-7-3-O-gallate ,catechin glyco-sidase ,piceatannol-3-O-β-D-(6″-O-galloyl)-glucopyranoside ,stilbene glucoside ,T HSG-glucuronide ,torachrysone-O-β-D-glucopyranoside ,emodin-8-O-(6′-O-malonyl)-β-D-glu-copyranoside , emodin , palmidin A and physcion , w hich presented different change laws .The results showed that stilbene glucoside ,torachrysone-O-β-D-glucopyranoside and emodin-8-O-(6′-O-malonyl )-β-D-glucopyranoside are much higher in the samples from Shibing county Guizhou province , 3 ,5-dihydroxyphenol-1-O-β-D-(6″-O-galloyl )-glucopyranoside ,gallic acid and palmidin A are much higher in the samples from Yings-han city Hubei province ,catechin glycosidase ,piceatannol-3-O-β-D-(6″-O-galloyl)-glu-copyranoside , T HSG-glucuronide and emodin are much higher in the samples from Gaozhou city Guangdong province ,pocyanidin B-7-3-O-gallate ,and physcion are much higher in the samples from A'ba county Sichuan province .This study can provide basic information for revealing the influence law of the different origin on the biosynthesis of metabolites in Polygoni Multifori Radix .关键词
何首乌/超高效液相色谱-串联四极杆飞行时间高分辨质谱(UPLC-TripleTOFMS/MS)/差异性化学成分/产地Key words
Polygoni Multifori Radix/UPLC-Triple TOF MS/MS/difference of chemical composition/habitats分类
化学化工引用本文复制引用
罗益远,刘娟秀,刘训红,王胜男,华愉教,兰才武,邢清清..基于UPLC-Triple TOF MS/MS技术分析不同产地何首乌的差异化学成分[J].质谱学报,2017,38(6):678-689,12.基金项目
国家科技支撑计划项目(2011BAI13B04) （2011BAI13B04）
江苏高校优势学科建设工程资助项目(ysxk-2014)资助 （ysxk-2014）
