郑州大学学报（理学版）2017，Vol.49Issue(4)：76-81,6.DOI:10.13705/j.issn.1671-6841.2017026
增强化学发光酶联免疫分析用于伏马菌素B1的快速检测
Enhanced Chemiluminescence Enzyme-linked Immunoassay for Rapid Detection of Fumonisin B1
摘要
Abstract
Based on the immune reaction between antigen and antibody and chemiluminescence , a new method for rapid detection of fumonisin B 1 was established using luminol-H2 O2 as luminescent substrates and the hydroxy diphenyl as chemiluminescence enhancer , and named as enhanced chemiluminescence enzyme-linked immunoassay(ECLEIA).On the basis of optimizing the experimental conditions , the limit of detection was 91.3 μg/L, and the linear range of the ECLEIA method for FB 1 determination was from 200 μg/L to 1400μg/L, while the correlation coefficient was 0.9916.Furthermore, the relative stand-ard deviations of intra-assay and inter-assay were 9.1%~10.2%( n=6 ) and 10.6%~12.4% ( n=6),respectively.The recoveries were 85.9%~115.4%, and the cross-reactivity rates with object ana-logues were low .Compared with other common detection methods ( HPLC and ELISA ) , ECLEIA method had high sensitivity , high specificity , sample operation and rapid detection , which could be used for the fast testing of large quantities of real samples .关键词
增强化学发光酶联免疫分析/伏马菌素B1/快速检测Key words
enhanced chemiluminescence enzyme-linked immunoassay/fumonisin B 1/rapid detection分类
化学化工引用本文复制引用
刘贝贝,于斐,玉崧成,刘利娥,吴拥军..增强化学发光酶联免疫分析用于伏马菌素B1的快速检测[J].郑州大学学报（理学版）,2017,49(4):76-81,6.基金项目
国家自然科学青年基金项目(81402721). （81402721）
