北京大学学报（自然科学版）2017，Vol.53Issue(6)：1115-1121,7.DOI:10.13209/j.0479-8023.2017.122
高效褐藻胶降解菌的筛选与产酶条件优化
Screening of Alginate Degrading Bacteria and Optimization of Its Enzyme-Producing Conditions
摘要
Abstract
53#, a strain of efficient alginate degrading bacteria, is isolated from rotted kelp using sodium alginate as the only carbon resource. Strain 53# is identified asPaenibacillusagaridevorans based on physiological characteristics and 16S rRNA sequencing. The optimaltion yield condition of strain 53# is identified at pH=8, T=25oC, NaCl 15 g/L, and sodium alginate 15 g/L by orthogonal experiment and analysis, and the highest enzyme activity is 390.53±17.32 U/mL. Strain 53# has the advantages such as being cultivated easily, producing enzyme fast and having high enzyme activity. It can achieve a high efficiency for saccharification of alginate and thus has potential value to be utilized in the production of bioethanol from brown algae.关键词
褐藻胶降解/褐藻胶裂解酶/类芽孢杆菌/生物乙醇Key words
alginate degradation/alginate lyase/Paenibacillus/biofuel分类
资源环境引用本文复制引用
李婷婷,陈倩,石萍,耿旭..高效褐藻胶降解菌的筛选与产酶条件优化[J].北京大学学报（自然科学版）,2017,53(6):1115-1121,7.基金项目
北京市科技专项(Z161100005016034)资助 （Z161100005016034）
