长春中医药大学学报2017，Vol.33Issue(6)：868-870,3.DOI:10.13463/j.cnki.cczyy.2017.06.003
藻蓝蛋白对家兔心肌缺血-再灌注损伤的影响
Effects of phycocyanin on myocardial ischemia reperfusion injury in rabbits
摘要
Abstract
Objective To investigate the effect of phycocyanin on myocardial ischemia-reperfusion injury in rabbits. Methods 60 rabbits were randomly numbered and divided into sham operation group, ischemia reperfusion group and phycocyanin groups1,2,3, myocardial ischemia-reperfusion injury models were established in rabbits except the sham operation group. After modeling for 2 h, the three phycocyanin groups were treated by different dose of phycocyanin suspension of 50, 200, 400 mg/ (kg?d) by gavage for 2 weeks; the sham operation group and ischemia reperfusion group were given the same volume of saline as control. The ELISA assay was used to test the level of MB isoenzyme of CK (CK-MB), nitric oxide (NO), superoxide dismutase (SOD) activity, endothelin -1 (ET-1), endothelial nitric oxide synthase (eNOS) and malondialdehyde (MDA) in rabbit serum; the positive expression of inducible nitric oxide synthase (iNOS) was detected by the immunohistochemical staining; reverse transcription polymerase chain reaction (RT-PCR) was used to detect the mRNA expression of vascular endothelial growth factor (VEGF) in ischemic regions. Results In the phycocyanin group 2 and group 3, the expression level of NO, SOD and eNOS significantly increased, and the number of iNOS positive cells also increased; in the ischemic tissues, the high expressions of VEGF can be detected, but the expression level of ET-1, CK-MB and MDA significantly decreased compared with the modelgroup (P < 0.05), the difference was statistically significant. Conclusion The phycocyanin has protective effects on myocardial ischemia reperfusion injury in rabbits.关键词
家兔/藻蓝蛋白/酶/缺血-再灌注损伤/血管内皮生长因子Key words
rabbit/phycocyanin/enzyme/ischemia-reperfusion injury/vascular endothelial growth factor分类
医药卫生引用本文复制引用
杨莎莎,周利..藻蓝蛋白对家兔心肌缺血-再灌注损伤的影响[J].长春中医药大学学报,2017,33(6):868-870,3.基金项目
湖北省科技厅资助项目(15Y29). （15Y29）
