长春中医药大学学报2017，Vol.33Issue(6)：886-889,4.DOI:10.13463/j.cnki.cczyy.2017.06.009
平喘颗粒对哮喘大鼠气道重塑后表皮生长因子蛋白表达的影响
Effect of Pingchuan granule on the expression of epidermal growth factor protein after airway remodeling in asthmatic rats
摘要
Abstract
Objective To investigate effect of Pingchuan granule on the expression of epidermal growth factor protein in asthmatic rats after airway remodeling by measuring the expression of epidermal growth factor protein after airway remodeling in asthmatic rats. Methods 90 Wistar rats were divided into 6 groups, 15 in each group, group A and group B were given normal saline by intragastric administration. Group C was inhaled with budesonide (0.5 mg of budesonide suspension, inhaled in compressed atomizer, twice daily), D, E and F were given low dose (4.01 g /kg/d), medium (8.01 g/kg/d) and high (16.02 g/kg/d). Each group was sacrificed 24 hours after the last excitation, and the lung tissue of the middle and lower part of the left lung was cut into 1mm × 1mm × 1mm size. The tissue was placed in an EP tube with 2.5% glutaraldehyde and fixed for electron microscopy. Take part of the right lung tissue, PBS rinse dry water, liquid nitrogen frozen, placed in -80 ℃ refrigerator for RT-PCR detection. Results The expression of EGF mRNA protein in (the low、medium、high) dose Pingchuan granule group and Budesonide group and model group was higher than that in the blank group, and the difference was statistically significant (P < 0.05). The expression of EGF mRNA protein in (the low, medium, high) dose Pingchuan granule group and budesonide group was significantly lower than that in model group (P < 0.05). The expression of EGF mRNA protein in (the medium, high) dose Pingchuan granule group was significantly lower than that in budesonide group but with no statis tical difference (P > 0.05). The expression of EGF mRNA protein in low dose Pingchuan granules group was lower than that in budesonide group, but the difference was not statistically significant. Conclusion Pingchuan granule can improve and control the airway remodeling process of airway hyperresponsiveness and airway obstruction status, and can inhibit the expression of EGF mRNA, thereby protecting the lung tissue cells, reduce asthma attacks.关键词
平喘颗粒/气道重塑/表皮生长因子Key words
Pingchuan granule/airway remodeling/epidermal growth factor分类
医药卫生引用本文复制引用
蒋鹏娜,刘建秋,李竹英,王雪慧..平喘颗粒对哮喘大鼠气道重塑后表皮生长因子蛋白表达的影响[J].长春中医药大学学报,2017,33(6):886-889,4.基金项目
国家自然科学基金青年科学基金项目(81403234) （81403234）
黑龙江省自然科学基金项目面上项目(H201481) （H201481）
黑龙江中医药大学 " 优秀创新人才支持计划 " 项目(051268) （051268）
黑龙江中医药大学新药研究基金项目(2014xy04) （2014xy04）
黑龙江省普通本科高等学校青年创新人才培养计划(UNPYSCT-2015120). （UNPYSCT-2015120）
