重庆医学2017，Vol.46Issue(33)：4630-4632,3.DOI:10.3969/j.issn.1671-8348.2017.33.008
对称性肢端角化病超微结构变化及角蛋白1的表达研究
Study on ultramicrostructure change and keratin1 expression in patients with symmetrical acral keratoderma
摘要
Abstract
Objective To study the ultramicrostructure change and keartin(KRT1) expression in skin lesion of symmetrical acral keratoderma(SAK) .Methods Thirteen cases of SAK in the First Affiliated Hospital of Fujian Medical University and the outpatient department of the Dongguan Municipal Sixth People′s Hospital were selected as the study subjects .The histopathological samples were taken from the wrist site .The retinoic acid preparation or corticosteroid preparation or Chinese medicine preparation were not externally used within 2 months before taking skin lesion sample .The healthy control skin samples were the normal skin in 12 cases by plastic surgical resection .The ultramicrostructural change were observed by the transmission electron microscopy .The KRT1 expression in skin lesion of 13 cases of SAK and healthy skin tissue of 12 cases were measured by immunohistochemistry method .Results The SAK ultramicrostructures manifested by the interruption of keratinizing envelope continuity in horny layer , and remarkable aggregation of keratin filament in upper stratum spinosum and surrounding nucleus of granular layer .KRT1 was ex-pressed in the cells of SAK skin lesion and basal layer ,spinous layer ,granular layer and horny layer .The cytoplasm and cytomem-brane staining was common .The KRT1 expression in skin lesion was significantly higher than that in normal skin (t=2 .210 ,P=0 .038) .Conclusion The ultramicrostructure features of SAK skin lesion are abnormal differentiation of epidermis keratin fila-ments ,which might be related with overexpression of KRT 1 .关键词
角蛋白1/对称性肢端角化病/超微结构Key words
keartin-1/symmetrical acral keratoderma/ultramicrostructure分类
生物科学引用本文复制引用
李荣华,李笑梅,孙娟娟,游晓意,庄永灿,李红杏,官飞凤,李常兴..对称性肢端角化病超微结构变化及角蛋白1的表达研究[J].重庆医学,2017,46(33):4630-4632,3.基金项目
广东省医学科学技术研究基金资助项目(A2016025) （A2016025）
东莞市社会科技发展资助项目(2014108101008) （2014108101008）
泉州市科技计划资助项目(2015Z39). （2015Z39）
