中国妇幼健康研究2017，Vol.28Issue(11)：1325-1327,3.DOI:10.3969/j.issn.1673-5293.2017.11.003
循环miRNA-210的表达与胎儿生长受限的相关性分析
Correlation analysis of circulating miRNA-210 expression level with fetal growth restriction
摘要
Abstract
Objective To study the relationship between miRNA-210 and fetal growth restriction(FGR)and clarify the effect of circulating miRNA-210 in the pathogenesis of FGR,so as to provide new biomolecular maker for early diagnosis and pathogenesis of FGR.Methods From January 2015 to January 2016 random principle was adopted to select 30 prenant women delivering full-term neonates with FGR in FGR group and 30 cases of normal controls in control group.RNA was extracted and Trizol reagent and Real Time-PCR were used to determine circulating miRNA-210 expression level and relative value of reference gene U 6 to analyze the correlation of miRNA-210 with FGR.Results There was no obvious difference between FGR group and normal control group in maternal age,pre-pregnancy weight and gestational age(t value was 0.84,0.74 and 0.48,respectively,all P>0.05),but neonatal weight of FGR group was remarkably lower than that of the control group with statistical difference(t=8.56,P<0.01).The level of circulating miRNA-210 was 4.58 in FGR group,which increased significantly compared to the control group(t= 7.43,P<0.01).MiRNA-210 might be used as a potential biological marker to distinguish neonates with FGR from normal ones.Conclusion The expression level of miRNA-210 is significantly increased in FGR group,and miRNA-210 may be involved in the pathogenesis of FGR.Circulating miRNA-210 can be used as biomarker in early diagnosis of FGR.关键词
胎儿生长受限/循环/生物标志物/miRNA-210Key words
fetal growth restriction(FGR)/circulation/biomarker/miRNA-210分类
医药卫生引用本文复制引用
丁桂春,李冰心,刘倩,张玲玲..循环miRNA-210的表达与胎儿生长受限的相关性分析[J].中国妇幼健康研究,2017,28(11):1325-1327,3.基金项目
扬州市科技计划项目(YZ2014214) （YZ2014214）
