摘要
Abstract
Objective To compare the chromosomal abnormalities in cleavage and blastocyst stages by analyzing the chromosome results of patients undergoing preimplantation genetic screening.Methods Patients undergoing preimplantation genetic screening (PGS)in reproductive center of Northwest Women and Children's Hospital from January 1,2015 to April 30,2017 were collected,and embryo biopsy was carried out when embryo was cultured to cleavage and blastocyst stage.Array-comparative genomic hybridization(array-CGH)(Blue Gnome 24SureV3)was adopted for PGS.Embryo chromosome analyzing results in blastocyst and cleavage stages were compared.Results A total of 348 embryos were examined in PGS,including 67 embryos in cleavage stage and 281 in blastocyst stage.Aneuploidy rate of embryo in blastocyst stage was higher than that in cleavage stage (43.68% vs 14.75%,P<0.01).It was most common in embyos in cleavage stage that more than 5 chromosomes were abnormal (42.31%)while one abnormal chromosome in blastocysts was most common(53.79%).In embryos in cleavage stage,incidence of abnormality in chromosome 20,21,16 and 14 was high(>5.00%),while that in chromosome 8,4 and 12 was low(<3.00%). Abnormal rate of sex chromosome 22,16,19,7 and 1 and chromosome 21 and 15 was high in blastocysts(> 5.00%),but abnormal rate of chromosome 10,17,12 and 3 was low(< 3.00%).For chromosome deletion abnormalities,incidence of chromosome deletion in chromosome 20,16,22,1 and 21 was high(8.14%,6.98%,6.98%,6.59% and 5.81%,respectively,all above 5.50%),and incidence of chromosome deletion was low in chromosome 3(0.78%).Incidence of chromosome duplication abnormality in sex chromosome 19,16,22 and 21 and chromosome 7,15,13 was high(>5.50%),and that in chromosome 12 was the lowest(1.46%).Conclusion Aneuploidy rate of embryo in blastocyst stage is higher than that in cleavage stage.It is most common in embryos in cleavage stage that more than 5 chromosomes are abnormal,and one abnormal chromosome in blastocyst is most common.Incidence of abnormality in chromosome 20,21,16 and 14 is high in embryos in cleavage stage,while abnormality in sex chromosome 22,16,19,7 and 1 and chromosome 21 and 15 is high in blastocyst.关键词
植入前遗传学筛查/卵裂期胚胎/囊胚/染色体异常/非整倍体Key words
preimplantation genetic screening(PGS)/embryos in cleavage stage/blastocyst/chromosomal abnormalities/aneuploidy分类
医药卫生
