南方农业学报2017，Vol.48Issue(11)：1923-1929,7.DOI:10.3969/j.issn.2095-1191.2017.11.01
木薯海藻糖合成酶基因MeTPS6克隆及其在非生物胁迫下的表达分析
Cloning of trehalose-6-phosphate synthase gene MeTPS6 from cassava and its expression under abiotic stress
摘要
Abstract
[Objective]Trehalose-6-phosphate synthase gene MeTPS6 from cassava was cloned,and its expression un-der abiotic stresses including drought,cold and shade was analyzed,in order to provide a reference for the study of TPS gene function and its anti-adversity molecular mechanism.[Method]RT-PCR method was applied to clone gene MeTPS6 from cassava leaves,bioinformatics analysis was carried out and a phylogenetic tree was constructed. Fluorescence quanti-tative PCR(qPCR)was used to analyze the expression characteristics and patterns of gene MeTPS6 in different cassava va-rieties and tissues under different abiotic stresses including drought,cold and shade.[Result]Cloned gene MeTPS6 had a 2565-bp open reading frame,encoded 854 amino acids,and contained a conserved TPS family domain(Glyco_transf_20). Protein MeTPS6 had close genetic relationship with Populus trichocarpa and Salix purpurea,its homologous sequence similarity with P. trichocarpa(Potri. 010G104500)and S. purpurea(SapurV1A. 0015s0610)was up to 92.6%and 90.0%respectively. Promoter sequence in gene MeTPS6 had a large number of abiotic stress related motifs(cold-related motif LTR and drought-related motif MBS),hormone related motifs(abscisic acid related motif ABRE and CE3,salicylic acid related motif TCA-element),and light response related motifs(ACE and Box I). Gene MeTPS6 expressed the highest in storage root,followed by fibrous root,and the expression in these two tissues were significantly higher than those in stem, petiole and leaf(P<0.05,the same below). The expression patterns of gene MeTPS6 were obviously different in various tissues under drought,cold and shade stresses,but its expression was significantly induced under these stresses.[Result]At a high confidence level(Confidence=0.8),thirteen co-expressed genes were found,among which ten were TPS homo-logous genes,suggesting these genes might coordinate with each other and participate in the process of plant biology, while the other three genes(CAT,CAT1,and F5M15.5)encoded catalase and mainly involved in the protection of cells from toxic effects of hydrogen peroxide.[Conclusion]Gene MeTPS6 mainly expresses in cassava root(especially storage root)and participate in the transcriptional regulation of response to drought,cold,and shade stress treatments. It can be served as a candidate to further studying its functions in cassava anti-adversity.关键词
木薯/海藻糖合成酶/MeTPS6基因/干旱/低温/遮荫/表达分析Key words
cassava/trehalose-6-phosphate synthase/gene MeTPS6/drought/cold/shade/expression analysis分类
农业科技引用本文复制引用
丁泽红,铁韦韦,付莉莉,颜彦,胡伟..木薯海藻糖合成酶基因MeTPS6克隆及其在非生物胁迫下的表达分析[J].南方农业学报,2017,48(11):1923-1929,7.基金项目
国家自然科学基金项目(31600198) （31600198）
海南省自然科学基金项目(20163120) （20163120）
