河北工业科技2017，Vol.34Issue(6)：434-439,6.DOI:10.7535/hbgykj.2017yx06008
奥贝胆酸有关物质的分析及合成
Synthesis and analysis of the related substances of obeticholic acid
摘要
Abstract
In order to control the quality of obeticholic acid,three related substances are separated from the obeticholic acid,as 3α-hydroxy-6-ethyl-7-keto-5β-cholene-24-acid (A ),3α,7α-dihydroxy-6-ethylidene-5β-cholene-24-acid (B ) and 3α(3α,7α-dihydroxy-6α-ethyl-5β-cholyl-24-acyloxy)-7α-hydroxy-6α-ethyl-5β-cholene-24-acid (C).The cause of the substances existence is analyzed and the route for preparing the substances is designed,and the structure of the substances are determined by 1 H NMR.The study shows that the relative retention time of the three related substances is consistent with that of the main related substances in obeticholic acid crude products,and the nuclear magnetic resonance hydrogen spectra of the synthesized related substances is consistent with that of the separated related substances,so it is determined that the main related substances in obeticholic acid products are the related substance A,related substance B and related substance C.The study solves the prob-lem of the reference substances needed for the study of the relative substances,improves the quality of the obeticholic acid,and provides some help for the quality research of obeticholic acid and control of the related substances.关键词
中药化学/奥贝胆酸/结构鉴定/有关物质/合成/质量控制Key words
Chinese medicine chemistry/obeticholic acid/structural identification/related substances/synthesis/control quality分类
医药卫生引用本文复制引用
范甜,史校东,张晓晓,刘洋,姚军..奥贝胆酸有关物质的分析及合成[J].河北工业科技,2017,34(6):434-439,6.基金项目
河北省人力资源和社会保障厅择优资助项目(C201400317) （C201400317）
