郑州大学学报（医学版）2017，Vol.52Issue(6)：668-672,5.DOI:10.13705/j.issn.1671-6825.2017.06.002
miR-199 a-3 p对Eca109和KYSE450细胞周期和迁移的影响
Effects of miR-199 a-3 p on cell cycle and migration of Eca109 and KYSE450 cells
摘要
Abstract
Aim:To study the effects and molecular mechanism of miR-199a-3p on cell cycle and migration in esopha-geal squamous cell carcinoma .Methods:miR-199a-3p mimics and miR-199a-3p mimics negative control were transfected into Eca109 and KYSE450 cells,and the expression level of miR-199a-3p in cells was detected by qRT-PCR.The cell cycle and migration were investigated by flow cytometry and wound healing assay , and the mTORC2 signaling pathway-related proteins such as mTOR,Rictor,PI3K,p-Akt(T308), and p-Akt(S473) were detected by Western blot.Results:The ex-pression of miR-199a-3p was significantly increased in two cell lines after transfected with miR-199a-3p mimics ( P <0.001).The results of cell cycle and wound healing assay showed that in cells transfected with miR -199a-3p mimics,the percentage of G1 phase cells was higher and the rate of cell migration was lower compared with those transfected with miR -199a-3p mimics negative control(P<0.05).Western blot results confirmed that the expression levels of mTOR and Rictor were obviously reduced, while those of PI3K,p-Akt(T308) and p-Akt(S473) were obviously increased in cells transfected with miR-199a-3p mimics than those transfected with miR-199a-3p mimics negative control(P<0.05).Conclusion:miR-199a-3p may affect the cell cycle and migration of esophageal squamous cell carcinoma cells through regulating the mTORC2 signaling pathway .关键词
miR-199a-3p/食管鳞癌/细胞周期/细胞迁移/Eca109/KYSE450Key words
miR-199a-3p/esophageal squamous cell carcinoma/cell cycle/cell migration/Eca109/KYSE450分类
医药卫生引用本文复制引用
刘襄艳,侯桂琴,高盼,凡丞,张幸丽,师莹莹,鲁照明..miR-199 a-3 p对Eca109和KYSE450细胞周期和迁移的影响[J].郑州大学学报（医学版）,2017,52(6):668-672,5.基金项目
国家自然科学基金资助项目 30901778 （）
河南省基础与前沿技术研究计划项目 162300410122 （）
