郑州大学学报（医学版）2017，Vol.52Issue(6)：677-680,4.DOI:10.13705/j.issn.1671-6825.2017.06.004
M2型巨噬细胞对食管癌移植瘤脉管生成的影响
Effect of M2 type macrophage on angiogenesis and lymphangiogenesis of human esophageal cancer xenograft
摘要
Abstract
Aim:To investigate the effect of M2 type macrophage on angiogenesis and lymphangiogenesis of human e-sophageal cancer xenograft .Methods:Human lymphoma U937 monocytes were induced into M 2 type macrophage by PMA and IL-4.The M2 type macrophage was co-cultured with esophageal cancer EC 9706 cells.Sixteen nude mice were allocated into two groups and were injected with co-culture M2 type macrophage with EC9706 cells(co-culture group) or single-culture EC9706 cells(control group).The formation of tumor in the 2 groups was observed.The mRNA expression levels of VEGF and VEGF-C in xenograft of the 2 groups were determined by in-situ hybridization method .The levels of VEGF and VEGF-C proteins in xenograft of the 2 groups were detected by immunohistochemical technique .The expression levels of CD 31 and D2-40 separately to mark the blood vessel and the lymphatic vessel were detected by immunohistochemistry , and the value of MVD and LMVD was calculated .Results: The protein and mRNA expression levels of VEGF and VEGF-C in co-culture group were significantly higher than those in control group (P<0.05).Both the value of MVD and that of LMVD in co-cul-ture group were also higher than those in control group (P<0.05).Conclusion:M2 type macrophage may promote angiogen-esis and lymphatic vessel generation of esophageal cancer xenograft in nude mice by secreting VEGF and VEGF -C.关键词
肿瘤相关巨噬细胞/食管癌/移植瘤/VEGF/VEGF-CKey words
tumor associated macrophage/esophageal carcinoma/xenograft/VEGF/VEGF-C分类
医药卫生引用本文复制引用
兰青,贺璐璐,韦娜,孙淼淼,陈金艳,杨建萍,王正洋,郑湘予,陈奎生..M2型巨噬细胞对食管癌移植瘤脉管生成的影响[J].郑州大学学报（医学版）,2017,52(6):677-680,4.基金项目
国家自然科学基金资助项目 81272370 （）
河南省基础与前沿技术研究计划项目 152300410026,122300413204 （）
