郑州大学学报（医学版）2017，Vol.52Issue(6)：697-701,5.DOI:10.13705/j.issn.1671-6825.2017.06.009
HDAC1基因沉默的hUC-MSCs移植对创伤性脑损伤小鼠的神经保护作用
Neural protective effect of transplantation of hUC-MSCs with silenced HDAC1 in traumatic brain injury mice
摘要
Abstract
Aim: To evaluate whether transplantation of hUC-MSCs with silenced HDAC1 could facilitate recovery of nerve function in the mice model of traumatic brain injury .Methods: Moderate traumatic brain injury mice models were made by the stereotaxic apparatus and modified weight-drop model.Eighty C57BL/6 mice were randomly allocated into four groups:Sham group, Vehicle group, hUC-MSCs group and Combination group (n=20).After brain injury, mice neurological function was evaluated by the modified neurological severity score on the 1st, 3rd, 7th, 14th, 21st and 28th day.The indexes of motor function and cognitive function were evaluated via the Morris water maze test , the tail suspension test and the novel object recognition , respectively .The size of cortical lesion and the morphological changes of neurons in hippocampal CA 3 area were detected by crystal violet staining .Brain water content was measured with wet-dry weight formula .Blood brain barrier permeability was measured by Evans blue staining .Results:Compared with Vehicle group , the modified neurological severi-ty scores significantly decreased in hUC-MSCs group and Combination group , the escape latency distinctly reduced accompa-nied with a sharp increase in both the times of crossing the plat and the time in target quadrant , time of wire hanging and dis-crimination index were increased, especially in Combination group(P<0.05).The evaluating indexes in Combination group such as brain damage volume , brain water content ,localized opening blood brain barrier and the loss of neurons in the hipp-ocampal CA3 area were appreciably reduced (P<0.05).Conclusion:Transplantation of hUC-MSCs with HDAC1 deficiency could provide a better neuroprotection in traumatic brain injury mice .关键词
HDAC1/基因沉默/hUC-MSC/创伤性脑损伤/小鼠/神经保护Key words
HDAC1/gene silencing/hUC-MSC/traumatic brain injury/mouse/neuroprotection分类
医药卫生引用本文复制引用
许玲,李鹏,关方霞,邢衢,马珊珊,程田,杨璐,黄团结,程康,刘雯雯,刘艳霞..HDAC1基因沉默的hUC-MSCs移植对创伤性脑损伤小鼠的神经保护作用[J].郑州大学学报（医学版）,2017,52(6):697-701,5.基金项目
国家自然科学基金资助项目U1404313,81471306,81601078 （）
河南省高校科技创新团队基金资助项目 15IRTSTHN022 （）
河南省科技创新人才计划基金资助项目 154200510008 （）
河南省国际人才合作项目 2016GH03,2016GH15 （）
河南省高等学校重点科研项目 17A310012 （）
