郑州大学学报（医学版）2017，Vol.52Issue(6)：702-706,5.DOI:10.13705/j.issn.1671-6825.2017.06.010
粪菌移植对阿尔茨海默病小鼠学习记忆能力的影响
Effects of fecal microbiota transplantation on learning and memory ability of Alzheimer′s disease mice
摘要
Abstract
Aim:To study the effects of fecal microbiota transplantation(FMT) on learning and memory ability and amyloidβ-protein(Aβ) deposition in Alzheimer′s disease mice.Methods:A total of 15 mice were randomly allocated into APP -group, APP+group, and FMT group(n=5).The APP+mice in FMT group were treated with FMT for 4 weeks, while the mice in APP+group and APP-group were enemaed with normal saline.The learning and memory ability of mice was tested by Morris water maze.And then Western blot was employed to detect the expression of senescence related proteins (P21, P53, Sirt1, Sirt2, PCNA) in mice brain tissue, and the change of Aβdeposition in the brain was observed by Congo red staining.Results:Com-pared with the APP-group, the escaping latency of the APP+group was longer, while the times of crossing the platform quad-rant was less and the time of spending in the target quadrant was shorter(P<0.05).After FMT, the escaping latency of the mice was decreased, the times of crossing the platform quadrant and the time of spending in the target quadrant were increased (P<0.05).And the deposition of Aβin brain tissue of FMT group was reduced compared with the APP +group(P<0.05).Addi-tionally, the expressions of P21 and P53 in brain tissue of FMT group reduced, and the expressions of Sirt1, Sirt2 and PCNA in-creased significantly(P<0.05).Conclusion:FMT could improve the learning and memory ability of Alzheimer′s disease mice by slowing down its aging and reducing the formation of Aβdeposition in brain.关键词
小鼠/阿尔茨海默病/粪菌移植/肠道菌群/β淀粉样蛋白Key words
mouse/Alzheimer's disease/fecal microbiota transplantation/gut microbiota/amyloid β-protein分类
医药卫生引用本文复制引用
杨璐,关方霞,李庆华,许玲,刘雯雯,刘艳霞,黄团结,邢衢,李鹏,张乐..粪菌移植对阿尔茨海默病小鼠学习记忆能力的影响[J].郑州大学学报（医学版）,2017,52(6):702-706,5.基金项目
国家自然科学基金资助项目 81471306 （）
河南省高校科技创新团队基金资助项目 15IRTSTHN022 （）
河南省科技创新人才计划基金资助项目154200510008 （）
