郑州大学学报（医学版）2017，Vol.52Issue(6)：706-710,5.DOI:10.13705/j.issn.1671-6825.2017.06.011
20(S)-原人参二醇通过调控miRNA-19b诱导乳腺癌MCF-7细胞凋亡的观察
Observation of 20 ( S)-protopanaxadiol-induced apoptosis of breast cancer MCF-7 cells via regulating miRNA-19 b
摘要
Abstract
Aim:To investigate the possible mechanism of 20(S)-protopanaxadiol[20(S)-PPD] which could induce apoptosis of breast cancer MCF-7 cells via regulating miRNA-19b.Methods:MCF-7 cells were treated with 30,40,50,60, 70,80 μmol/L 20(S)-PPD for 48 h and the cell viability was detected by MTT method .MCF-7 cells were treated with 30, 50,80μmol/L 20(S)-PPD for 48 h, the expression level of miRNA-19b was detected by real-time PCR, and DNMT activi-ty assay kit was used to detect the DNMT activity .MCF-7 cells were divided into four groups: miRNA mimics plus blank medium group, miRNA-19b mimics plus 20(S)-PPD group, miRNA-19b mimics plus blank medium group, and miRNA mimics plus 20(S)-PPD group.The expression of miRNA-19b in the four groups was detected by real-time PCR.The pro-tein expression level of TNFαin the four groups was detected by Western blot .The cell apoptosis in the four groups was tested by flow cytometry .Results: 20 ( S )-PPD could suppress the viability of MCF-7 cells concentration-dependently . 20(S)-PPD could significantly decrease the expression of miRNA-19b with the increase of the concentration (P<0.05).80μmol/L 20(S)-PPD significantly enhanced the activity of DNMT (P<0.05).After transfecting miRNA-19b mimics, the expression of miRNA-19b upregulated,the expression of TNFαwas repressed, and the apoptosis rate reduced (P<0.05). After joining 65 μmol/L 20(S)-PPD, the expression of miRNA-19b decreased,the expression of TNFαincreased, and the apoptosis rate increased(P<0.05).Conclusion: In MCF-7 cells, 20(S)-PPD may inhibit the expression of miRNA-19b, increase the expression of TNFα, and induce apoptosis through its methylation role .关键词
MCF-7细胞/20(S)-原人参二醇/miRNA-19b/TNFα/凋亡Key words
MCF-7 cell/20(S)-protopanaxadiol/miRNA-19b/TNFα/apoptosis分类
医药卫生引用本文复制引用
杨晓艳,覃熙媛,李楠,罗志勇,杨芳..20(S)-原人参二醇通过调控miRNA-19b诱导乳腺癌MCF-7细胞凋亡的观察[J].郑州大学学报（医学版）,2017,52(6):706-710,5.基金项目
国家自然科学基金资助项目 81101655 （）
湖南省自然科学基金资助项目 12JJ3099 （）
