郑州大学学报（医学版）2017，Vol.52Issue(6)：749-753,5.DOI:10.13705/j.issn.1671-6825.2017.06.021
番泻叶水提物对3 T3-L1前脂肪细胞增殖、凋亡及脂联素基因表达的影响
Effect of Folium Sennae aqueous extracts on 3 T3-L1 cell apoptosis and expression of adipokines
摘要
Abstract
Aim:To determine the effect of Folium Sennae aqueous extract ( FSAE) on 3T3-L1 cell proliferation and apoptosis, and further study its effect on 3T3-L1 adiponectin mRNA and adiponectin promoter luciferase activity .Methods:3T3-L1 cells were incubated with different concentrations of FSAE for 24 h, and cell proliferation was determined by MTT method;AnnexinV-FITC/PI double staining was used to detect cells apoptosis;Western blot was used to detect the expres-sions of Caspase-3 and Bax protein;Fluorescence quantitative PCR was used to detect adiponectin mRNA expression ;doub-le fluorescein enzyme report gene method was used to detect the influence of adiponectin gene promoter activity .Results:Different concentration FSAE inhibited 3T3-L1 proliferation,promoted cell apoptosis , improved the expressions of apoptosis related protein Caspase-3 and Bax;At the same time , FSAE improved adiponectin mRNA expression , and enhanced its adi-ponectin promoter activity .Conclusion:This may provide a molecular basis for FASE inhibiting obesity .关键词
番泻叶水提物/3T3-L1前脂肪细胞/荧光素酶Key words
Folium Sennae aqueous extract/3T3-L1 cell/luciferase activity分类
医药卫生引用本文复制引用
刘卫红,崔琳,路玲玲,李强..番泻叶水提物对3 T3-L1前脂肪细胞增殖、凋亡及脂联素基因表达的影响[J].郑州大学学报（医学版）,2017,52(6):749-753,5.基金项目
河南省教育厅自然科学研究资助计划项目 2010B360009 （）
郑州市科技攻关计划项目 0910SGYS33391-1 （）
